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Product Info Summary
| SKU: | A14387 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-TWA1 GID8 Antibody
SKU/Catalog Number
A14387
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-TWA1 GID8 Antibody (Catalog # A14387). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
TWA1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-TWA1 GID8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14387)
Host
Rabbit
Contents
TWA1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
TWA1 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human TWA1. The immunogen is located within the last 50 amino acids of TWA1.
Reactive Species
A14387 is reactive to GID8 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
26.7 kDa
Background of GID8
TWA1 was identified through a two hybrid-associated protein screen with RanBPM. TWA1 is well conserved through evolution and is localized within the nucleus. It interacts with RanBP9 and comprises a protein complex with RanBPM and Muskelin. TWA1 was found to possess the LisH-CTLH motif which is detected in proteins involved in microtubule dynamics, cell migration, nucleokinesis and chromosome segregation. These functions overlap with functions suggested for the RanGTPase cycle. Recent findings suggested that there is an as yet uncovered function of the RanGTPase cycle.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14387 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
TWA1 antibody can be used for detection of TWA1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of TWA1 in human brain tissue lysate with TWA1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunofluorescence of TWA1 in rat brain tissue with TWA1 antibody at 20 μg/mL.
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Immunohistochemistry of TWA1 in rat brain tissue with TWA1 antibody at 2.5 μg/mL.
Specific Publications For Anti-TWA1 GID8 Antibody (A14387)
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