Anti-TYW2 TRMT12 Antibody

Product Name

Anti-TYW2 TRMT12 Antibody

View all TRMT12 Antibodies

SKU/Catalog Number

A15532

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-TYW2 TRMT12 Antibody (Catalog # A15532). Tested in ELISA, WB, ICC applications. This antibody reacts with Human, Mouse.

Storage & Handling

TYW2 antibody can be stored at 4 °C, stable for one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-TYW2 TRMT12 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15532)

Host

Rabbit

Contents

TYW2 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

TYW2 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human TYW2. The immunogen is located within the first 50 amino acids of TYW2.

Cross-reactivity

TYW2 antibody is predicted to not cross-react with other TYW protein family members. At least two isoforms of TYW2 are known to exist; this antibody will detect both isoforms

Reactive Species

A15532 is reactive to TRMT12 in Human, Mouse

Observed Molecular Weight

68 kDa

Calculated molecular weight

50.2 kDa

Background of TRMT12

TYW2, also known as TRM12, is an enzyme that participates in the wybutosine-tRNA (Phe) biosynthesis pathway. Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TYW2 is involved in a multistep enzymatic reaction that stabilizes codon-anticodon base-pairing during the ribosomal decoding process, thereby ensuring correct translation. TYW2 was found to be amplified in 7 of 8 breast cancers cell lines analyzed, and qPCR analysis of 30 breast tumors showed overexpression of TYW2 mRNA of over 2-fold in 26, suggesting that TYW2 may play a role in breast cancer.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A15532 is guaranteed for ELISA, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

TYW2 antibody can be used for detection of TYW2 by Western blot at 0.5 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information TRMT12

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