TRMT12 Antibodies

AND TRMT12 ELISA kits, TRMT12 Proteins

Many biological assays use antibodies to detect TRMT12, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with TRMT12 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop TRMT12 antibodies...

We validate the specificity of these antibodies to TRMT12 by testing them on tissues known to express TRMT12 positively and negatively. Browse below to find the TRMT12 antibody that suites your experiment. We have 4 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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TRMT12 Infographic by Boster Bio

All TRMT12 Products

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Specificity

Applications

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Anti-TYW2 TRMT12 Antibody

Western blot analysis of TYW2 in K562 cell lysate with TYW2 antibody at 0

Immunocytochemistry of TYW2 in K562 cells with TYW2 antibody at 10 μg/mL

Human, Mouse

ELISA, ICC, WB

$481
Cat # A15532
0.1 mg

Anti-TRMT12 Mouse Monoclonal Antibody [Clone ID: OTI1C9]

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRMT12 (Right lane) cDNA for 48 hrs and lysed

Human

IHC, WB

$523
Cat # M15532-1
100 µl

Anti-TRMT12 Mouse Monoclonal Antibody [Clone ID: OTI5C9]

Western blot analysis of extracts (35ug) from HepG2 cell lines by using anti-TRMT12 monoclonal antibody (1:500)

Human

IHC, WB

$523
Cat # M15532
100 µl

TRMT12 (NM_017956) Human Over-expression Lysate

Human

$628
Cat # LS055039
20 µg, 100 µg

TRMT12 Overview

Facts about tRNA wybutosine-synthesizing protein 2 homolog.

TRMT12 3D structure. Source: alphafold

tRNA wybutosine-synthesizing protein 2 homolog (TRMT12)

S-adenosyl-L-methionine-dependent transferase that serves as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine using a tricyclic base located at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA.

Catalyzes the transfer of the alpha-amino-alpha- carboxypropyl (acp) group from S-adenosyl-L-methionine to the C-7 position of 4-demethylwyosine (imG-14) to produce wybutosine-86. .

Gene Information

Human
Gene Name:	TRMT12
Uniprot:	Q53H54
Entrez:	55039

Family

Belongs to:

class I-like SAM-binding methyltransferase superfamily

Alternative Names

Alpha-amino-alpha-carboxypropyl transferase TYW2; EC 2.1.1; EC 2.1.1.32; EC 2.5.1.-; FLJ20772; homolog of yeast tRNA methyltransferase; TRM12; tRNA methyltranferase 12 homolog (S. cerevisiae); tRNA methyltranferase 12 homolog; tRNA methyltransferase 12 homolog (S. cerevisiae); tRNA-yW synthesizing protein 2; tRNA-yW-synthesizing protein 2; TYW2tRNA wybutosine-synthesizing protein 2 homolog

Molecular Weight

Mass (kDA):
50.236 kDA

Genomic Context


Human

Location:	8q24.13
Sequence:	8; NC_000008.11 (124450820..124453026)

Diseases

Cell Transformation, Neoplastic
Malignant Neoplasms
Neoplasms
Malignant Neoplasm Of Breast
Mammary Neoplasms

Interacting Proteins

LNX1

Pathways

Segmentation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/TRMT12

References

PMID: 22761755 by Rodriguez V., et al. Structure-function analysis of human TYW2 enzyme required for the biosynthesis of a highly modified Wybutosine (yW) base in phenylalanine-tRNA.