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Product Info Summary
| SKU: | A15532 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, ICC, WB |
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Product info
Product Name
Anti-TYW2 TRMT12 Antibody
SKU/Catalog Number
A15532
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-TYW2 TRMT12 Antibody (Catalog # A15532). Tested in ELISA, WB, ICC applications. This antibody reacts with Human, Mouse.
Storage & Handling
TYW2 antibody can be stored at 4 °C, stable for one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-TYW2 TRMT12 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15532)
Host
Rabbit
Contents
TYW2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
TYW2 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human TYW2. The immunogen is located within the first 50 amino acids of TYW2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
TYW2 antibody is predicted to not cross-react with other TYW protein family members. At least two isoforms of TYW2 are known to exist; this antibody will detect both isoforms
Reactive Species
A15532 is reactive to TRMT12 in Human, Mouse
Applications
A15532 is guaranteed for ELISA, ICC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
50.236kDa
Background of TRMT12
TYW2, also known as TRM12, is an enzyme that participates in the wybutosine-tRNA (Phe) biosynthesis pathway. Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TYW2 is involved in a multistep enzymatic reaction that stabilizes codon-anticodon base-pairing during the ribosomal decoding process, thereby ensuring correct translation. TYW2 was found to be amplified in 7 of 8 breast cancers cell lines analyzed, and qPCR analysis of 30 breast tumors showed overexpression of TYW2 mRNA of over 2-fold in 26, suggesting that TYW2 may play a role in breast cancer.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
TYW2 antibody can be used for detection of TYW2 by Western blot at 0.5 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of TYW2 in K562 cell lysate with TYW2 antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunocytochemistry of TYW2 in K562 cells with TYW2 antibody at 10 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For TRMT12 (Source: Uniprot.org, NCBI)
Gene Name
TRMT12
Full Name
tRNA wybutosine-synthesizing protein 2 homolog
Weight
50.236kDa
Superfamily
class I-like SAM-binding methyltransferase superfamily
Alternative Names
Alpha-amino-alpha-carboxypropyl transferase TYW2; EC 2.1.1; EC 2.1.1.32; EC 2.5.1.-; FLJ20772; homolog of yeast tRNA methyltransferase; TRM12; tRNA methyltranferase 12 homolog (S. cerevisiae); tRNA methyltranferase 12 homolog; tRNA methyltransferase 12 homolog (S. cerevisiae); tRNA-yW synthesizing protein 2; tRNA-yW-synthesizing protein 2; TYW2tRNA wybutosine-synthesizing protein 2 homolog TRMT12 TRM12, TYW2 tRNA methyltransferase 12 homolog tRNA wybutosine-synthesizing protein 2 homolog|alpha-amino-alpha-carboxypropyl transferase TYW2|homolog of yeast tRNA methyltransferase|tRNA methyltranferase 12 homolog|tRNA(Phe) (4-demethylwyosine(37)-C(7)) aminocarboxypropyltransferase|tRNA-yW-synthesizing protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TRMT12, check out the TRMT12 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TRMT12: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TYW2 TRMT12 Antibody (A15532)
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No publications found for A15532
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Customer Reviews
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