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Product Info Summary
| SKU: | A14797 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-TYW4 LCMT2 Antibody
SKU/Catalog Number
A14797
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-TYW4 LCMT2 Antibody (Catalog # A14797). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
TYW4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-TYW4 LCMT2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14797)
Host
Rabbit
Contents
TYW4 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
TYW4 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human TYW4. The immunogen is located within the first 50 amino acids of TYW4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
TYW4 antibody is predicted to not cross-react with other TYW protein family members. At least two isoforms of TYW4 are known to exist; this antibody will detect only the long isoform.
Reactive Species
A14797 is reactive to LCMT2 in Human, Mouse, Rat
Applications
A14797 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
75.602kDa
Background of LCMT2
TYW4 is an enzyme that participates in the wybutosine-tRNA (Phe) biosynthesis pathway. Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TYW4 is involved in a multistep enzymatic reaction that stabilizes codon-anticodon base-pairing during the ribosomal decoding process, thereby ensuring correct translation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
TYW4 antibody can be used for detection of TYW4 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of TYW4 in rat brain tissue lysate with TYW4 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of TYW4 in mouse brain tissue with TYW4 antibody at 5 μg/mL.
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Immunofluorescence of TYW1 in mouse brain tissue with TYW1 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For LCMT2 (Source: Uniprot.org, NCBI)
Gene Name
LCMT2
Full Name
tRNA wybutosine-synthesizing protein 4
Weight
75.602kDa
Superfamily
methyltransferase superfamily
Alternative Names
EC 2.1.1.-; FLJ51321; KIAA0547tRNA-Wybutosine-synthesizing protein 4; leucine carboxyl methyltransferase 2; MGC9534; p21WAF1/CIP1 promoter-interacting protein; PPM2; tRNA wybutosine-synthesizing protein 4 homolog; tRNA yW-synthesizing protein 4 homolog; TYW4tRNA-yW synthesizing protein 4 LCMT2 PPM2, TYW4 leucine carboxyl methyltransferase 2 tRNA wybutosine-synthesizing protein 4|p21WAF1/CIP1 promoter-interacting protein|tRNA(Phe) (7-(3-amino-3-(methoxycarbonyl)propyl)wyosine(37)-N)-methoxycarbonyltransferase|tRNA(Phe) (7-(3-amino-3-carboxypropyl)wyosine(37)-O)-methyltransferase|tRNA-Wybutosine-synthesizing protein 4|tRNA-yW synthesizing protein 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LCMT2, check out the LCMT2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LCMT2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TYW4 LCMT2 Antibody (A14797)
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No publications found for A14797
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Customer Reviews
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