Product Info Summary
SKU: | A02810-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-UBA1 Antibody
View all Ubiquitin-activating Enzyme/UBE1 Antibodies
SKU/Catalog Number
A02810-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-UBA1 Antibody catalog # A02810-1. Tested in WB,ICC/IF,IHC,Flow Cytometry applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-UBA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02810-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from Internal of human PIAS3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02810-1 is reactive to UBA1 in Human, Mouse, Rat
Applications
A02810-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
117.849kDa
Background of Ubiquitin-activating Enzyme/UBE1
Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth.
Jiang-Hong Man, Nucleic Acids Res., Nov 2006; 34: 5552 - 5566.
Amir Sonnenblick, Mol. Cell. Biol., Dec 2004; 24: 10584 - 10592.
Jianyin Long, PNAS, Jan 2004; 101: 99.
Hyun Duk Jang, J. Biol. Chem., Jun 2004; 279: 24873 - 24880.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000-1:2,000
ICC: 1:50-1:200
IHC: 1:100-1:500
FC: 1:50-1:100
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunocytochemistry staining of UBA1 using Anti-UBA1 Antibody (A02810-1).
ICC staining UBA1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehydeClick image to see more details
Figure 1. Western blotting validation for Anti-UBA1 Antibody A02810-1
Western blot analysis of UBA1 on mouse liver lysates using anti-UBA1 antibody at 1/5Protein Target Info & Infographic
Gene/Protein Information For UBA1 (Source: Uniprot.org, NCBI)
Gene Name
UBA1
Full Name
Ubiquitin-like modifier-activating enzyme 1
Weight
117.849kDa
Superfamily
ubiquitin-activating E1 family
Alternative Names
A1S9T and BN75 temperature sensitivity complementing; A1S9T; A1ST; AMCX1; GXP1; MGC4781; POC20 centriolar protein homolog; POC20; Protein A1S9; SMAX2; UBA1; UBA1, ubiquitin-activating enzyme E1 homolog A; UBA1A; UBE1; UBE1A1S9; UBE1X; ubiquitin-activating enzyme E1 (A1S9T and BN75 temperature sensitivitycomplementing); Ubiquitin-activating enzyme E1; Ubiquitinactivating Enzyme; Ubiquitin-activating Enzyme; ubiquitin-like modifier activating enzyme 1; ubiquitin-like modifier-activating enzyme 1 UBA1 A1S9, A1S9T, A1ST, AMCX1, CFAP124, GXP1, POC20, SMAX2A, UBE1, UBE1X, VEXAS, UBA1 ubiquitin like modifier activating enzyme 1 ubiquitin-like modifier-activating enzyme 1|A1S9T and BN75 temperature sensitivity complementing|POC20 centriolar protein homolog|UBA1, ubiquitin-activating enzyme E1 homolog A|testicular secretory protein Li 63
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on UBA1, check out the UBA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for UBA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-UBA1 Antibody (A02810-1)
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No publications found for A02810-1
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