Anti-UBE2T Antibody

Product Name

Anti-UBE2T Antibody

View all UBE2T Antibodies

SKU/Catalog Number

A05874

Size

100ul

Form

Liquid

Description

Boster Bio Anti-UBE2T Antibody catalog # A05874. Tested in WB applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-UBE2T Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05874)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human DLL3 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A05874 is reactive to UBE2T in Human, Mouse, Rat

Applications

A05874 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

22.521kDa

Background of UBE2T

Deubiquitinating enzyme that regulates the degradation of various proteins. Deubiquitinates and prevents proteasomal degradation of RNF123 which in turn stimulates CDKN1B ubiquitin-dependent degradation thereby playing a role in cell proliferation. Involved in decreased protein synthesis in atrophying skeletal muscle. Modulates transcription of major myofibrillar proteins. Also involved in turnover of endoplasmic-reticulum-associated degradation (ERAD) substrates. Regulates the stability of BIRC2/c-IAP1 and BIRC3/c-IAP2 by preventing their ubiquitination. Required for cells to mount an appropriate response to hypoxia and rescues HIF1A from degradation in a non-catalytic manner. Plays an important role in 17 beta-estradiol (E2)-inhibited myogenesis. Decreases the levels of ubiquitinated proteins during skeletal muscle formation and acts to repress myogenesis. Exhibits a preference towards 'Lys-63'-linked Ubiquitin chains.

Nagase T., DNA Res. 5:355-364(1998).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Beausoleil S.A., Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:1000

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blotting validation for Anti-UBE2T Antibody A05874

Western blot (WB) analysis of UBE2T polyclonal antibody at 1:500 dilution
Lane1:MCF-7 whole cell lysate(40ug)
Lane2:HepG2 whole cell lysate(40ug)
Lane3:CT26 whole cell lysate(40ug)
Lane4:C6 whole cell lysate(40ug)
Lane5:H9C2 whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For UBE2T (Source: Uniprot.org, NCBI)

Alternative Names

Cell proliferation-inducing gene 50 protein; EC 6.3.2.19; HSPC150 protein similar to ubiquitin-conjugating enzyme; HSPC150; PIG50; UBE2T; Ubiquitin carrier protein T; ubiquitin conjugating enzyme; ubiquitin-conjugating enzyme E2 T; ubiquitin-conjugating enzyme E2T (putative); Ubiquitin-protein ligase T UBE2T FANCT, HSPC150, PIG50 ubiquitin conjugating enzyme E2 T ubiquitin-conjugating enzyme E2 T|E2 ubiquitin-conjugating enzyme T|HSPC150 protein similar to ubiquitin-conjugating enzyme|cell proliferation-inducing gene 50 protein|ubiquitin carrier protein T|ubiquitin conjugating enzyme E2T|ubiquitin-conjugating enzyme E2T (putative)|ubiquitin-protein ligase T

For more info on UBE2T, check out the UBE2T Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for UBE2T: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the UBE2T Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis