Anti-Ubiquilin1 Antibody (Center)

Product Name

Anti-Ubiquilin1 Antibody (Center)

View all Ubiquilin 1 Antibodies

SKU/Catalog Number

A00823

Size

80 µl

Form

Liquid

Description

Boster Bio Anti-Ubiquilin1 Antibody (Center) (Catalog # A00823). Tested in IF, WB, IHC-P application(s). This antibody reacts with Human, Mouse.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-Ubiquilin1 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00823)

Host

Rabbit

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

This Ubiquilin1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 296-326 amino acids from the Central region of human Ubiquilin1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A00823 is reactive to UBQLN1 in Human, Mouse

Applications

A00823 is guaranteed for IF, IHC-P, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

62.519kDa

Background of Ubiquilin 1

Ubiquilin 1 (UBQLN1), also known as DA41, was isolated from an adult rat lung cDNA library, and encodes a cellular protein that associates with DAN.1 DAN expression is reduced in rat fibroblast 3Y1 cells transformed with mouse sarcoma virus and in rodent fibroblasts transformed with a variety of oncogenes. The DAN-DA41 interaction is mediated through the N-terminal domain and a cysteine-knot region of DAN. Human DA41 encodes a 589-amino acid protein with 86% amino acid sequence identity with rat protein.2 DA41 expression is regulated in a cell cycle-dependent manner. PLIC1 and PLIC2 (UBQLN2) are homologs of the mouse Plics (proteins linking integrin-associated protein (IAP) and cytoskeleton) and the yeast Dsk2 protein. PLIC1, also called UBQLN1, shares 72% amino acid identity with PLIC2,3 Two motifs are conserved in the mammalian PLICs and yeast Dsk2, an N-terminal ubiquitin-like (UBL) domain and a C-terminal ubiquitin-associated (UBA) domain. Unlike ubiquitin, the UBL domain of the PLICs does not have a diglycine motif in its C terminus. The UBA domain is present in multiple enzyme classes of the ubiquitination machinery. Human PLICs associate with both proteasomes and ubiquitin ligases in large complexes. Overexpression of PLICs impairs the in vivo degradation of 2 unrelated ubiquitin-dependent proteasome substrates, p53 and I-kappa-B-alpha (NFKBIA), but not a ubiquitin-independent substrate. PLICs may link the ubiquitination machinery to the proteasome to affect in vivo protein degradation. The DA41 gene maps to chromosome 9q21.2-q21.3, a position overlapping a candidate tumor suppressor locus for bladder cancer.2

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

IF: 1:100
WB: 1:1000
IHC-P: 1:50-1:100

Validation Images & Assay Conditions

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Immunofluorescent analysis of Hela cells, using Ubiquilin1 Antibody (Center) . A00823 was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor® 554 (red) conjugated Phalloidin (red).

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Ubiquilin1 Antibody (P311) (Cat. # A00823) western blot analysis in SH-SY5Y cell line lysates (35ug/lane). This demonstrates the Ubiquilin1 antibody detected the Ubiquilin1 protein (arrow).

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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Gene/Protein Information For UBQLN1 (Source: Uniprot.org, NCBI)

Alternative Names

DA41; DA41FLJ90054; DSK2; hPLIC-1; PLIC1; PLIC-1; PLIC-1UBQN; Protein linking IAP with cytoskeleton 1; Ubiquilin 1; ubiquilin-1; UBQLN1; UBQN; XDRP1 UBQLN1 DA41, DSK2, PLIC-1, UBQN, XDRP1 ubiquilin 1 ubiquilin-1|hPLIC-1|protein linking IAP with cytoskeleton 1|testicular tissue protein Li 219

For more info on UBQLN1, check out the UBQLN1 Infographic

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In this infographic, you will see the following information for UBQLN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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