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Product Info Summary
| SKU: | A00060 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB, IHC-P-Leica |
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Product info
Product Name
Anti-Ubiquitin Antibody (N-term)
View all Ubiquitin C Antibodies
SKU/Catalog Number
A00060
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-Ubiquitin Antibody (N-term) (Catalog # A00060). Tested in IHC-P-Leica, WB application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-Ubiquitin Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00060)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This Ubiquitin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-32 amino acids from the N-terminal region of human Ubiquitin.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00060 is reactive to UBC in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
77039 Da
Background of Ubiquitin C
This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00060 is guaranteed for WB, IHC-P-Leica Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC-P-Leica: 1:500
Validation Images & Assay Conditions
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All lanes : Anti-Ubiquitin Antibody (N-term) at 1:2000 dilution
Lane 1: 293 whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: HepG2 whole cell lysate
Lane 4: A549 whole cell lysate
Lane 5: NIH/3T3 whole cell lysate
Lane 6: C2C12 whole cell lysate
Lane 7: C6 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 10 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded Human kidney tissue using A00060 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
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Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue using A00060 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Protein Target Info & Infographic
Gene/Protein Information For UBC (Source: Uniprot.org, NCBI)
Gene Name
UBC
Full Name
Polyubiquitin-C
Weight
77039 Da
Superfamily
ubiquitin family
Alternative Names
Polyubiquitin-C, Ubiquitin, UBC UBC HMG20 ubiquitin C polyubiquitin-C
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on UBC, check out the UBC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for UBC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ubiquitin Antibody (N-term) (A00060)
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