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Product Info Summary
| SKU: | A00820 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Ubiquitin-like protein ATG12 ATG12 Antibody
SKU/Catalog Number
A00820
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Ubiquitin-like protein ATG12 ATG12 Antibody (Catalog # A00820). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
ATG12 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Ubiquitin-like protein ATG12 ATG12 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00820)
Host
Rabbit
Contents
ATG12 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ATG12 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human ATG12. The immunogen is located within the first 50 amino acids of ATG12.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody is predicted to have no cross-reactivity to ACVR1 or ACVR1B.
Reactive Species
A00820 is reactive to ATG12 in Human, Mouse, Rat
Applications
A00820 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
15.113kDa
Background of ATG12
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG12, another member of the autophagy protein family, forms a conjugate with ATG5; this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells. ATG12 has also been shown to interact with ATG10 in human embryonic kidney cells in the presence of ATG7. At least two isoforms of ATG12 are known to exist.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ATG12 antibody can be used for the detection of ATG10 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ATG12 in human brain tissue lysate with ATG12 antibody at (A) 0.5
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Immunohistochemistry of ATG12 in human brain tissue with ATG12 antibody at 2.5 μg/mL.
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Immunofluorescence of ATG12 in Human Brain cells with ATG12 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For ATG12 (Source: Uniprot.org, NCBI)
Gene Name
ATG12
Full Name
Ubiquitin-like protein ATG12
Weight
15.113kDa
Superfamily
ATG12 family
Alternative Names
Apg12 (autophagy 12, S. cerevisiae)-like; APG12 autophagy 12-like (S. cerevisiae); APG12; APG12HAPG12; APG12-like; ATG12 autophagy related 12 homolog (S. cerevisiae); ATG12; Autophagy-related protein 12; FBR93; HAPG12; ubiquitin-like protein ATG12; yeast) homolog ATG12 APG12, APG12L, FBR93, HAPG12 autophagy related 12 ubiquitin-like protein ATG12|APG12 autophagy 12-like|ATG12 autophagy related 12 homolog|Apg12 (autophagy, yeast) homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATG12, check out the ATG12 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATG12: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ubiquitin-like protein ATG12 ATG12 Antibody (A00820)
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No publications found for A00820
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