Anti-UBP43 USP18 Antibody

Product Name

Anti-UBP43 USP18 Antibody

View all USP18 Antibodies

SKU/Catalog Number

A04028

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-UBP43 USP18 Antibody (Catalog # A04028). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-UBP43 USP18 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04028)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 25-50 of human UBP43 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A04028 is reactive to USP18 in Human

Applications

A04028 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

43.011kDa

Background of USP18

UBP43 (also known as ISG43, Ubl thiolesterase 18, ISG15-specific processing protease, 43 kDa ISG15-specific protease, ubiquitin specific protease 18 or USP18) is a member of the deubiquitinating protease family of enzymes, which removes ubiquitin adducts from a broad range of protein substrates. Deletion of the UBP43 gene in mouse leads to a massive increase of ISG15 conjugates in tissues indicating that UBP43 is a major ISG15-specific protease. UBP43 is the first ISG15-specific protease reported. Both ISG15 and UBP43 genes are known to be strongly induced by interferon, genotoxic stress, and viral infection. Thus UBP43 may be necessary to maintain a critical cellular balance of ISG15-conjugated proteins in both healthy and stressed organisms.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000 - 1:50,000
IF Microscopy: User optimized
WB: 1:500 - 1:2,000

Validation Images & Assay Conditions

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Western blot analysis of UBP43 expression in HELA nuclear extracts (lane 1), HELA whole cell lysates (lane 2), A431 whole cell lysates (lane 3), JURKAT whole cell lysates (lane 4) and HEK293 whole cell lysates (lane 5). UBP43 at 43KD was detected using rabbit anti-UBP43 Antigen Affinity purified polyclonal antibody (Catalog # A04028) at 1:500. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).

Gene/Protein Information For USP18 (Source: Uniprot.org, NCBI)

Alternative Names

EC 3.1.2.15; EC 3.4.19.-; hUBP43; ISG15-specific-processing protease; ISG43; ISG43UBP43; ubiquitin specific peptidase 18; ubiquitin specific protease 18; ubl carboxyl-terminal hydrolase 18; ubl thioesterase 18,43 kDa ISG15-specific protease; Ubl thiolesterase 18; UBP43; USP18 USP18 ISG43, PTORCH2, UBP43 ubiquitin specific peptidase 18 ubl carboxyl-terminal hydrolase 18|43 kDa ISG15-specific protease|ISG15-specific-processing protease|hUBP43|ubiquitin specific protease 18|ubl thioesterase 18|ubl thiolesterase 18

For more info on USP18, check out the USP18 Infographic

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In this infographic, you will see the following information for USP18: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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