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Product Info Summary
| SKU: | M05812 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-UBR2 Picoband® Antibody (monoclonal, 2G10)
SKU/Catalog Number
M05812
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-UBR2 Picoband® Antibody (monoclonal, 2G10) catalog # M05812. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-UBR2 Picoband® Antibody (monoclonal, 2G10) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05812)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Monoclonal
Clone Number
2G10
Isotype
Mouse IgG2a
Immunogen
E.coli-derived human UBR2 recombinant protein (Position: Q10-A664).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M05812 is reactive to UBR2 in Human
Observed Molecular Weight
202 kDa
Calculated molecular weight
200.5 kDa
Background of UBR2
E3 ubiquitin-protein ligase UBR2 is an enzyme that in humans is encoded by the UBR2 gene. It is mapped to 6p21.1. This gene encodes an E3 ubiquitin ligase of the N-end rule proteolytic pathway that targets proteins with destabilizing N-terminal residues for polyubiquitylation and proteasome-mediated degradation. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05812 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human
Positive Control
WB: human HEK293 whole cell, human Jurkat whole cell, human K562 whole cell
Validation Images & Assay Conditions
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Western blot analysis of UBR2 using anti-UBR2 antibody (M05812).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human K562 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-UBR2 antigen affinity purified monoclonal antibody (Catalog # M05812) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for UBR2 at approximately 202KD. The expected band size for UBR2 is at 202KD.
Specific Publications For Anti-UBR2 Picoband® Antibody (monoclonal, 2G10) (M05812)
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