Product Info Summary
SKU: | M04276 |
---|---|
Size: | 80 µl |
Reactive Species: | Human |
Host: | Mouse |
Application: | WB |
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Product info
Product Name
Anti-UCH37 (UCHL5) Antibody
View all UCH-L5/UCH37 Antibodies
SKU/Catalog Number
M04276
Size
80 µl
Description
Boster Bio Anti-UCH37 (UCHL5) Antibody (Catalog # M04276). Tested in WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-UCH37 (UCHL5) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04276)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
854CT5.5.6
Isotype
IgG1
Immunogen
Purified His-tagged UCH37 protein was used to produced this monoclonal antibody.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M04276 is reactive to UCHL5 in Human
Applications
M04276 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
37.607kDa
Background of UCH-L5/UCH37
Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Putative regulatory component of the INO80 complex; however is inactive in the INO80 complex and is activated by a transient interaction of the INO80 complex with the proteasome via ADRM1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000-1:4000
Validation Images & Assay Conditions
Click image to see more details
All lanes : Anti-UCH37 (UCHL5) at 1:2000-1:4000 dilution
Lane 1: 293 whole cell lysate
Lane 2: NCI-H460 whole cell lysate
Lane 3: HeLa whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 37 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Protein Target Info & Infographic
Gene/Protein Information For UCHL5 (Source: Uniprot.org, NCBI)
Gene Name
UCHL5
Full Name
Ubiquitin carboxyl-terminal hydrolase isozyme L5
Weight
37.607kDa
Superfamily
peptidase C12 family
Alternative Names
CGI-70; EC 3.4.19.12; INO80 complex subunit R; INO80R; ubiquitin carboxyl-terminal esterase L5; ubiquitin carboxyl-terminal hydrolase isozyme L5; ubiquitin carboxyl-terminal hydrolase L5; Ubiquitin C-terminal hydrolase UCH37; Ubiquitin thioesterase L5; UCH37; UCH37CGI-70; UCHL5; UCH-L5 UCHL5 CGI-70, INO80R, UCH-L5, UCH37 ubiquitin C-terminal hydrolase L5 ubiquitin carboxyl-terminal hydrolase isozyme L5|INO80 complex subunit R|ubiquitin C-terminal hydrolase UCH37|ubiquitin carboxyl-terminal esterase L5|ubiquitin carboxyl-terminal hydrolase L5|ubiquitin thioesterase L5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on UCHL5, check out the UCHL5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for UCHL5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-UCH37 (UCHL5) Antibody (M04276)
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No publications found for M04276
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