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Product Info Summary
| SKU: | M04276 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-UCH37 (UCHL5) Antibody
SKU/Catalog Number
M04276
Size
80 µl
Description
Boster Bio Anti-UCH37 (UCHL5) Antibody (Catalog # M04276). Tested in WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-UCH37 (UCHL5) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04276)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
854CT5.5.6
Isotype
IgG1
Immunogen
Purified His-tagged UCH37 protein was used to produced this monoclonal antibody.
Reactive Species
M04276 is reactive to UCHL5 in Human
Calculated molecular weight
37.6 kDa
Background of UCHL5
Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Putative regulatory component of the INO80 complex; however is inactive in the INO80 complex and is activated by a transient interaction of the INO80 complex with the proteasome via ADRM1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04276 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000-1:4000
Validation Images & Assay Conditions
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All lanes : Anti-UCH37 (UCHL5) at 1:2000-1:4000 dilution
Lane 1: 293 whole cell lysate
Lane 2: NCI-H460 whole cell lysate
Lane 3: HeLa whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 37 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-UCH37 (UCHL5) Antibody (M04276)
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