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UGT1A6 antibody (A02239-1) performs well in ELISA, IF, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IF analysis of UGT1A6 using anti-UGT1A6 antibody (A02239-1).
UGT1A6 was detected in immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-UGT1A6 Antibody (A02239-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
UGT1A6 was detected in immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-UGT1A6 Antibody (A02239-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-UGT1A6 Antibody Picoband®
UGT1A6 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, IF, ICC, WB |
Tested Applications
UGT1A6 antibody was positively detected by WB in:
human HEK293 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
UGT1A6 antibody was positively detected by ICC/IF in:
U2OS cell
Recommend Dilution
Application
Dilution
Species
"Western blot
0.25-0.5μg/ml
Human
Immunocytochemistry/Immunofluorescence
2μg/ml
Human
ELISA
0.1-0.5μg/ml
-
"
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
ELISA
Human
Tested
Recommend
Monkey
Predicted
Predicted
Product Information
Product Name
Anti-UGT1A6 Antibody Picoband®
View all UGT1A6 Antibodies
View all UGT1A6 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human UGT1A6 recombinant protein (Position: D27-S251).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
55-61 kDa
Calculated Molecular Weight
60.8 kDa
Background of UGT1A6
UDP-glucuronosyltransferase 1-6 is an enzyme that in humans is encoded by the UGT1A6 gene. It is mapped to 2q37.1. This gene encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons, resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter. The enzyme encoded by this gene is active on phenolic and planar compounds. Alternative splicing in the unique 5' end of this gene results in two transcript variants.
Cite This Product
Anti-UGT1A6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02239-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
94%
High Homology
Dog
77%
Moderate Homology
Rabbit
75%
Moderate Homology
Cow
73%
Moderate Homology
Rat
73%
Moderate Homology
Mouse
71%
Moderate Homology
Drosophila
27%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for UGT1A6 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for UGT1A6 antibody
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Product Promise
We promise Anti-UGT1A6 Antibody Picoband® performs excellently in relevant applications for ELISA, IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A02239-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 7, 2026