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- Table of Contents
UGT1A6 · Western blot design guide
UGT1A6 Western Blot Planning Guide
Plan an UGT1A6 Western blot around the catalog-observed 60.8 kDa band, image-backed A02239-1 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
UGT1A6 Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 60.8 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 12-15% | |
| Positive control | Kidney | |
| Negative control | Appendix |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 60.8 kDa |
| ⓘ | Localization | Microsome / Endoplasmic reticulum membrane |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human |
Section 1
Real Curated UGT1A6 Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Kidney |
| Gel % | 12-15% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, standard transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A02239-1 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected UGT1A6 Western Blot Band Size?
Use the product-observed 60.8 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 60.8 kDa | Matches the authoritative product WB observation. |
| 60.8 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected UGT1A6 appearancePlan around 60.8 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 60.8 kDa; use this as the primary experimental expectation. |
| Calculated mass | 60.8 kDa from UniProt P19224; retain as context. |
| Gel selection | 12-15%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A02239-1. |
| Situation | Likely cause | Next action |
|---|---|---|
| 60.8 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing UGT1A6 band
A02239-1 · UGT1A6
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for UGT1A6 Western blot
🧪Use Kidney as the first positive-control candidate and Appendix as the HPA Not detected negative candidate.
Positive control: Kidney (High)
Negative control: Appendix (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for UGT1A6
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Kidney | Reported tissue cells | High | Protein (HPA) | HPA → |
| Urinary bladder | Reported tissue cells | High | Protein (HPA) | HPA → |
| Liver | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Stomach | Reported tissue cells | Medium | Protein (HPA) | HPA → |
Section 3
Advanced UGT1A6 Western Blot Tips
Deeper troubleshooting and optimisation questions for UGT1A6, answered from its protein features.
Which band should guide the blot?
Use 60.8 kDa, the observation attached to the authoritative A02239-1 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 60.8 kDa expectation.
Which positive control should I start with?
Start with Kidney, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Appendix as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 12-15% consistently across the quick facts, protocol table, and poster.
What transfer method to use for UGT1A6 Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A02239-1 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
UGT1A6 Western Blot Reagents
Human-reactive UGT1A6 Western blot reagents with authoritative product imagery.
Real WB data
Anti-UGT1A6 Antibody Picoband®
Cat # A02239-1
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt P19224
- Human Protein Atlas — UGT1A6
- A02239-1 product record
- PMC10794929 — Bile duct ligation elevates 5-HT levels in cerebral cortex of rats partly due to impairment of brain UGT1A6 expression and activity via ammonia accumulation (Redox biology, 2024)
- PMC6170201 — Role for protein kinase C delta in the functional activity of human UGT1A6: implications for drug-drug interactions between PKC inhibitors and UGT1A6 (Xenobiotica; the fate of foreign compounds in biological systems, 2010)
- PMC7700423 — Differential Effects of 1α,25-Dihydroxyvitamin D(3) on the Expressions and Functions of Hepatic CYP and UGT Enzymes and Its Pharmacokinetic Consequences In Vivo (Pharmaceutics, 2020)
