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Product Info Summary
| SKU: | A00584 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-ULK1 Antibody
SKU/Catalog Number
A00584
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ULK1 Antibody (Catalog # A00584). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
ULK1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-ULK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00584)
Host
Rabbit
Contents
ULK1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ULK1 antibody was raised against a 16 amino acid peptide near the center of human ULK1 . The immunogen is located within amino acids 350 - 400 of ULK1.
Cross-reactivity
Two alternatively spliced transcript variants encoding different isoforms have been identified. ULK1 antibody is predicted to not cross-react with ULK2.
Reactive Species
A00584 is reactive to ULK1 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
112.6 kDa
Background of ULK1
ULK1, also known as ATG1, is a key serine/threonine protein kinase probably acting at the most upstream step of autophagosome formation. Knockout of ULK1 results in a severe defect in the autophagy pathway. ULK1 is highly conserved among eukaryotes, and are the Unc-51-like kinases, ULK1 and ULK2 in mammals. ULK1 is ubiquitously expressed and involved in autophagy in response to starvation. It is the target of the TOR kinase signaling pathway that regulates autophagy through the control of phosphorylation status of ATG13. ULK1 also plays a role early in neuronal differentiation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00584 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Recommend Dilution
ULK1 Antibody can be used for detection of ULK1 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ULK1 in rat brain tissue lysate with ULK1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of ULK1 in rat brain tissue with ULK1 antibody at 2.5 μg/ml.
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Immunofluorescence of ULK1 in rat brain tissue with ULK1 antibody at 20 μg/ml.
Specific Publications For Anti-ULK1 Antibody (A00584)
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