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Product Info Summary
| SKU: | M07706-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-ULK3 Antibody (C-term)
SKU/Catalog Number
M07706-1
Size
80 µl
Description
Boster Bio Anti-ULK3 Antibody (C-term) (Catalog # M07706-1). Tested in WB application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-ULK3 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M07706-1)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1404CT148.89.25
Isotype
IgG1,k
Immunogen
This ULK3 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 435-468 amino acids from the C-terminal region of human ULK3.
Reactive Species
M07706-1 is reactive to ULK3 in Human, Mouse, Rat
Calculated molecular weight
53.4 kDa
Background of ULK3
Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M07706-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC: 1:50
WB: 1:1000
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded Human kidney section using Pink1. M07706-1 was diluted at 1:50 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
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Immunohistochemical analysis of paraffin-embedded Human kidney section using Pink1. M07706-1 was diluted at 1:50 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary
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Western blot analysis of lysates from Hela, LNCaP cell line, mouse testis and rat testis tissue lysates(from left to right), using ULK3 Antibody (C-term). M07706-1 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35μg per lane.
Specific Publications For Anti-ULK3 Antibody (C-term) (M07706-1)
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