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Product Info Summary
| SKU: | M07706 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-ULK3 Monoclonal Antibody
SKU/Catalog Number
M07706
BM5324 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-ULK3 Monoclonal Antibody catalog # M07706. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ULK3 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M07706)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ACHC-21
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ULK3 Serine/threonine protein kinase which enhances GLI1 and GLI2 transcriptional activity and consequently positively regulates GLI-dependent SHH signaling. May exert this function by promoting GLI1 nuclear localization. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently.
Reactive Species
M07706 is reactive to ULK3 in Human, Mouse, Rat
Observed Molecular Weight
53 kDa
Calculated molecular weight
53.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M07706 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:20
Positive Control
WB: 293T cell
Validation Images & Assay Conditions
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Western blot analysis of ULK3 expression in 293T cell lysate.
Specific Publications For Anti-ULK3 Monoclonal Antibody (M07706)
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3 Customer Q&As for Anti-ULK3 Monoclonal Antibody
Question
Would M07706 anti-ULK3 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
J. Anderson
Verified customer
Asked: 2019-12-20
Answer
It shows on the product datasheet, M07706 anti-ULK3 Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-20
Question
Is a blocking peptide available for product anti-ULK3 Monoclonal antibody (M07706)?
Verified Customer
Verified customer
Asked: 2019-03-06
Answer
We do provide the blocking peptide for product anti-ULK3 Monoclonal antibody (M07706). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-03-06
Question
We are interested in to test anti-ULK3 Monoclonal antibody M07706 on mouse testis for research purposes, then I may be interested in using anti-ULK3 Monoclonal antibody M07706 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-07-26
Answer
The products we sell, including anti-ULK3 Monoclonal antibody M07706, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-07-26
