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Product Info Summary
| SKU: | M09436 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-UQCRH Rabbit Monoclonal Antibody
SKU/Catalog Number
M09436
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-UQCRH Rabbit Monoclonal Antibody catalog # M09436. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-UQCRH Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M09436)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
27U84
Isotype
IgG
Immunogen
A synthesized peptide derived from human UQCRH
Reactive Species
M09436 is reactive to UQCRH in Human, Mouse, Rat
Observed Molecular Weight
13 kDa
Calculated molecular weight
10.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M09436 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
Positive Control
WB: human K562 whole cell, human THP-1 whole cell, rat brain tissue, rat stomach tissue, mouse brain tissue, mouse stomach tissue
Validation Images & Assay Conditions
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Western blot analysis of UQCRH using anti-UQCRH antibody (M09436).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human THP-1 whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: rat stomach tissue lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse stomach tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UQCRH antigen affinity purified monoclonal antibody (Catalog # M09436) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UQCRH at approximately 13 kDa. The expected band size for UQCRH is at 11 kDa.
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