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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
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Anti-Urokinase Rabbit Monoclonal Antibody
Boster Bio Anti-Urokinase Rabbit Monoclonal Antibody catalog # M04352. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Urokinase Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04352)
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
A synthesized peptide derived from human Urokinase
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
M04352 is reactive to PLAU in Human, Mouse, Rat
M04352 is guaranteed for IHC, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Validation Images & Assay Conditions
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Western blot analysis of Urokinase expression in LnCaP cell lysate (M04352).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLAU monoclonal antibody (Catalog # M04352) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLAU
Gene/Protein Information For PLAU (Source: Uniprot.Org, NCBI)
Urokinase-type plasminogen activator
peptidase S1 family
ATF; EC 3.4.21; EC 184.108.40.206; plasminogen activator, urokinase; PLAU; uPA; u-PA; uPlasminogen Activator; u-Plasminogen Activator; urinary; Urokinase; urokinase-type plasminogen activator PLAU ATF, BDPLT5, QPD, UPA, URK, u-PA plasminogen activator, urokinase urokinase-type plasminogen activator|U-plasminogen activator|plasminogen activator, urinary*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on PLAU, check out the PLAU Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for PLAU: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for M04352
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