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- Table of Contents
PLAU antibody (M04352) performs well in IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 5 Publications
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IHC analysis of PLAU using anti-PLAU antibody (M04352).
PLAU was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PLAU Antibody (M04352) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
PLAU was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PLAU Antibody (M04352) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-Urokinase Rabbit Monoclonal Antibody
PLAU antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IHC, WB |
Tested Applications
PLAU antibody was positively detected by WB in:
LnCaP cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Urokinase Rabbit Monoclonal Antibody
View all PLAU Antibodies
View all PLAU Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AABC-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Urokinase
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
49 kDa
Calculated Molecular Weight
48.5 kDa
Cite This Product
Anti-Urokinase Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04352)
Protocols
Western Blot protocol for PLAU antibody
Publications
Application:
Species:
Expression of urokinase plasminogen activator and plasminogen activator inhibitor type-1 in ovarian cancer and its clinical significance
Published: 2012-11-20
Journal: ONCOLOGY REPORTS
Application: IHC
Impact Factor: 1.84
Species: Human
PMID: 23174953
Relationships of uPA and VEGF Expression in Esophageal Cancer and Microvascular Density with Tumorous Invasion and Metastasis
Published: 2012-07-31
Journal: Asian Pacific Journal of Cancer Prevention
Application: IHC
Impact Factor: 0.66
Species: Human
PMID: 22994764
Invasion-associated genes identified by gene expression profiling in extranodal natural killer/T-cell lymphoma, nasal type
Published: 2012-07-09
Journal: LEUKEMIA & LYMPHOMA
Application: IHC
Impact Factor: 2.58
Species: Human
PMID: 22680768
Effects of shRNA targeting maspin on invasion of gastric carcinoma SGC7901 cell line
Published: 2011-01-01
Journal: ONCOLOGY REPORTS
Application: WB
Impact Factor: 1.69
Species: Human
Effects of Guiyuanfang and autologous transplantation of bone marrow stem cells on rats with liver fibrosis
Published: 2005-02-28
Journal: WORLD JOURNAL OF GASTROENTEROLOGY
Application: IHC
Impact Factor: 0.00
Species: Rat
PMID: 15754396
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Product Promise
We promise Anti-Urokinase Rabbit Monoclonal Antibody performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M04352
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 10, 2026