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Product Info Summary
| SKU: | M03786 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-USP10 Monoclonal Antibody
SKU/Catalog Number
M03786
BM5386 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-USP10 Monoclonal Antibody catalog # M03786. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-USP10 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03786)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ADCB-21
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human USP10 Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, SNX3 and CFTR. Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53.
Reactive Species
M03786 is reactive to USP10 in Human
Observed Molecular Weight
110 kDa
Calculated molecular weight
87.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03786 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
Positive Control
WB: A375 cell
Validation Images & Assay Conditions
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Western blot analysis of USP10 expression in A375 cell lysate.
Specific Publications For Anti-USP10 Monoclonal Antibody (M03786)
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Customer Q&As
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6 Customer Q&As for Anti-USP10 Monoclonal Antibody
Question
Do you have a BSA free version of anti-USP10 Monoclonal antibody M03786 available?
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-USP10 Monoclonal antibody M03786 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-16
Question
Will anti-USP10 Monoclonal antibody M03786 work on zebrafish IP with bone marrow?
Verified Customer
Verified customer
Asked: 2020-03-13
Answer
Our lab technicians have not validated anti-USP10 Monoclonal antibody M03786 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-USP10 Monoclonal antibody M03786 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish bone marrow in IP, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-13
Question
Is a blocking peptide available for product anti-USP10 Monoclonal antibody (M03786)?
Verified Customer
Verified customer
Asked: 2020-01-20
Answer
We do provide the blocking peptide for product anti-USP10 Monoclonal antibody (M03786). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-01-20
Question
I was wanting to use your anti-USP10 Monoclonal antibody for IP for human brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain identification?
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
You can see on the product datasheet, M03786 anti-USP10 Monoclonal antibody has been tested for IP, IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-09
Question
We are currently using anti-USP10 Monoclonal antibody M03786 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on primate tissues as well?
R. Banerjee
Verified customer
Asked: 2018-02-23
Answer
The anti-USP10 Monoclonal antibody (M03786) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-23
Question
Is this M03786 anti-USP10 Monoclonal antibody reactive to the isotypes of USP10?
Verified Customer
Verified customer
Asked: 2017-10-02
Answer
The immunogen of M03786 anti-USP10 Monoclonal antibody is A synthesized peptide derived from human USP10 Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, SNX3 and CFTR. Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-10-02
