Anti-USP10 Antibody Picoband™

Product Name

Anti-USP10 Antibody Picoband™

View all USP10 Antibodies

SKU/Catalog Number

A03786-3

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-USP10 Antibody Picoband™ catalog # A03786-3. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-USP10 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03786-3)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human USP10 recombinant protein (Position: M1-L798).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A03786-3 is reactive to USP10 in Human, Mouse, Rat

Applications

A03786-3 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

110 kDa, 130 kDa

Calculated molecular weight

87.134kDa

Background of USP10

Ubiquitin specific peptidase 10, also known as USP10, is an enzyme which in humans is encoded by the USP10 gene. Ubiquitin is a highly conserved protein that is covalently linked to other proteins to regulate their function and degradation. This gene encodes a member of the ubiquitin-specific protease family of cysteine proteases. The enzyme specifically cleaves ubiquitin from ubiquitin-conjugated protein substrates. The protein is found in the nucleus and cytoplasm. It functions as a co-factor of the DNA-bound androgen receptor complex, and is inhibited by a protein in the Ras-GTPase pathway. The human genome contains several pseudogenes similar to this gene. Several transcript variants, some protein-coding and others not protein-coding, have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of USP10 using anti-USP10 antibody (A03786-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: human K562 whole cell lysates,
Lane 6: human SIHA whole cell lysates,
Lane 7: rat brain tissue lysates,
Lane 8: mouse brain tissue lysates,
Lane 9: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-USP10 antigen affinity purified polyclonal antibody (Catalog # A03786-3) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for USP10 at approximately 110,130 kDa. The expected band size for USP10 is at 87 kDa.

Gene/Protein Information For USP10 (Source: Uniprot.org, NCBI)

Alternative Names

Deubiquitinating enzyme 10; EC 3.4.19.12; KIAA0190MGC2621; ubiquitin carboxyl-terminal hydrolase 10; ubiquitin specific peptidase 10; ubiquitin specific protease 10; ubiquitin thioesterase 10; Ubiquitin Thiolesterase 10; Ubiquitin-specific-processing protease 10; UBPO; Uchrp; USP10 USP10 UBPO ubiquitin specific peptidase 10 ubiquitin carboxyl-terminal hydrolase 10|deubiquitinating enzyme 10|ubiquitin specific protease 10|ubiquitin thioesterase 10|ubiquitin thiolesterase 10|ubiquitin-specific-processing protease 10

For more info on USP10, check out the USP10 Infographic

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In this infographic, you will see the following information for USP10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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