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Product Info Summary
| SKU: | M05225-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-USP11 Antibody (C-term R565)
SKU/Catalog Number
M05225-1
Size
80 µl
Description
Boster Bio Anti-USP11 Antibody (C-term R565) (Catalog # M05225-1). Tested in Flow Cytometry, WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-USP11 Antibody (C-term R565) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05225-1)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1220CT620.193.189
Isotype
IgG1,κ
Immunogen
This USP11 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 32-300 amino acids from the N-terminal region of human USP11.
Reactive Species
M05225-1 is reactive to USP11 in Human
Calculated molecular weight
109.8 kDa
Background of USP11
Protease that can remove conjugated ubiquitin from target proteins and polyubiquitin chains. Inhibits the degradation of target proteins by the proteasome. Plays a role in the regulation of pathways leading to NF-kappa-B activation. Plays a role in the regulation of DNA repair after double-stranded DNA breaks.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05225-1 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
FC: 1:25
Validation Images & Assay Conditions
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Western blot analysis of lysates from Jurkat, Hela, LNCaP cell line (from left to right) using USP11 Antibody (N-term). M05225-1 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.
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Flow cytometric analysis of Hela cells using USP11 Antibody (C-term R565)(green, ) compared to an isotype control of mouse IgG1(blue). M05225-1 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.
Specific Publications For Anti-USP11 Antibody (C-term R565) (M05225-1)
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