Product Info Summary
SKU: | M05225-1 |
---|---|
Size: | 80 µl |
Reactive Species: | Human |
Host: | Mouse |
Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-USP11 Antibody (C-term R565)
SKU/Catalog Number
M05225-1
Size
80 µl
Description
Boster Bio Anti-USP11 Antibody (C-term R565) (Catalog # M05225-1). Tested in Flow Cytometry, WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-USP11 Antibody (C-term R565) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05225-1)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1220CT620.193.189
Isotype
IgG1,κ
Immunogen
This USP11 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 32-300 amino acids from the N-terminal region of human USP11.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M05225-1 is reactive to USP11 in Human
Applications
M05225-1 is guaranteed for Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
109.817kDa
Background of USP11
Protease that can remove conjugated ubiquitin from target proteins and polyubiquitin chains. Inhibits the degradation of target proteins by the proteasome. Plays a role in the regulation of pathways leading to NF-kappa-B activation. Plays a role in the regulation of DNA repair after double-stranded DNA breaks.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
FC: 1:25
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of lysates from Jurkat, Hela, LNCaP cell line (from left to right) using USP11 Antibody (N-term). M05225-1 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.
Click image to see more details
Flow cytometric analysis of Hela cells using USP11 Antibody (C-term R565)(green, ) compared to an isotype control of mouse IgG1(blue). M05225-1 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.
Protein Target Info & Infographic
Gene/Protein Information For USP11 (Source: Uniprot.org, NCBI)
Gene Name
USP11
Full Name
Ubiquitin carboxyl-terminal hydrolase 11
Weight
109.817kDa
Superfamily
peptidase C19 family
Alternative Names
Deubiquitinating enzyme 11; EC 3.1.2.15; EC 3.4.19.12; ubiquitin carboxyl-terminal hydrolase 11; ubiquitin specific peptidase 11; ubiquitin specific protease 11; Ubiquitin Thioesterase 11; Ubiquitin thiolesterase 11; ubiquitin-specific processing protease 11; Ubiquitin-specific-processing protease 11; UHX1; UHX1ubiquitin carboxyl-terminal hydrolase, X-linked; USP11 USP11 UHX1 ubiquitin specific peptidase 11 ubiquitin carboxyl-terminal hydrolase 11|deubiquitinating enzyme 11|ubiquitin carboxyl-terminal hydrolase, X-linked|ubiquitin thioesterase 11|ubiquitin thiolesterase 11|ubiquitin-specific processing protease 11
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on USP11, check out the USP11 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for USP11: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-USP11 Antibody (C-term R565) (M05225-1)
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