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Product Info Summary
| SKU: | M05225 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, ICC, WB |
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Product info
Product Name
Anti-USP11 Monoclonal Antibody
SKU/Catalog Number
M05225
BM5387 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-USP11 Monoclonal Antibody catalog # M05225. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-USP11 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05225)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ADCC-21
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human USP11 Protease that can remove conjugated ubiquitin from target proteins and polyubiquitin chains. Inhibits the degradation of target proteins by the proteasome. Plays a role in the regulation of pathways leading to NF-kappa-B activation. Plays a role in the regulation of DNA repair after double-stranded DNA breaks.
Reactive Species
M05225 is reactive to USP11 in Human, Mouse, Rat
Observed Molecular Weight
110 kDa
Calculated molecular weight
109.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05225 is guaranteed for Flow Cytometry, IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
IP 1:20
FC 1:20
Validation Images & Assay Conditions
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
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Western blot analysis of USP11 using anti-USP11 antibody (M05225).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human U20S whole cell lysates,
Lane 5: rat testis tissue lysates,
Lane 6: rat brain tissue lysates,
Lane 7: mouse testis tissue lysates,
Lane 8: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-USP11 antigen affinity purified monoclonal antibody (Catalog # M05225) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for USP11 at approximately 110 kDa. The expected band size for USP11 is at 110 kDa.
Specific Publications For Anti-USP11 Monoclonal Antibody (M05225)
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Customer Q&As
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6 Customer Q&As for Anti-USP11 Monoclonal Antibody
Question
Does anti-USP11 Monoclonal antibody M05225 work for WB with muscle?
Verified Customer
Verified customer
Asked: 2020-03-10
Answer
According to the expression profile of muscle, USP11 is highly expressed in muscle. So, it is likely that anti-USP11 Monoclonal antibody M05225 will work for WB with muscle.
Boster Scientific Support
Answered: 2020-03-10
Question
Is a blocking peptide available for product anti-USP11 Monoclonal antibody (M05225)?
Verified Customer
Verified customer
Asked: 2019-08-28
Answer
We do provide the blocking peptide for product anti-USP11 Monoclonal antibody (M05225). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-08-28
Question
We want to test anti-USP11 Monoclonal antibody M05225 on human muscle for research purposes, then I may be interested in using anti-USP11 Monoclonal antibody M05225 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
The products we sell, including anti-USP11 Monoclonal antibody M05225, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-20
Question
We are currently using anti-USP11 Monoclonal antibody M05225 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-01
Answer
The anti-USP11 Monoclonal antibody (M05225) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-01
Question
Can you help my question with product M05225, anti-USP11 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-07-03
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M05225 anti-USP11 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-07-03
Question
See below the WB image, lot number and protocol we used for muscle using anti-USP11 Monoclonal antibody M05225. Please let me know if you require anything else.
T. Parker
Verified customer
Asked: 2016-07-19
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-07-19
