Anti-USP22 Monoclonal Antibody

USP22 antibody

Boster Bio Anti-USP22 Monoclonal Antibody catalog # M03887. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.

Product Info Summary

SKU: M03887
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry, IP, IF, ICC, WB

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Product Name

Anti-USP22 Monoclonal Antibody

View all USP22 Antibodies

SKU/Catalog Number

M03887

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-USP22 Monoclonal Antibody catalog # M03887. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-USP22 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03887)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ADCG-21

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human USP22 Histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. Catalyzes the deubiquitination of both histones H2A and H2B, thereby acting as a coactivator.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M03887 is reactive to USP22 in Human

Applications

M03887 is guaranteed for Flow Cytometry, IP, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

29 kDa

Calculated molecular weight

59.961kDa

Background of USP22

Plays a major role in tight junction-specific obliteration of the intercellular space.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:50
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For USP22 (Source: Uniprot.org, NCBI)

Gene Name

USP22

Full Name

Ubiquitin carboxyl-terminal hydrolase 22

Weight

59.961kDa

Superfamily

peptidase C19 family

Alternative Names

Deubiquitinating enzyme 22; EC 3.1.2.15; EC 3.4.19.12; KIAA1063ubiquitin carboxyl-terminal hydrolase 22; KIAA1064; ubiquitin specific peptidase 22; ubiquitin specific peptidase 3-like; ubiquitin specific protease 22; Ubiquitin Thioesterase 22; Ubiquitin thiolesterase 22; ubiquitin-specific processing protease 22; Ubiquitin-specific-processing protease 22; USP22; USP3L USP22 USP3L ubiquitin specific peptidase 22 ubiquitin carboxyl-terminal hydrolase 22|deubiquitinating enzyme 22|ubiquitin specific protease 22|ubiquitin thioesterase 22|ubiquitin thiolesterase 22|ubiquitin-specific processing protease 22

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on USP22, check out the USP22 Infographic

USP22 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for USP22: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-USP22 Monoclonal Antibody

Question

Is this M03887 anti-USP22 Monoclonal antibody reactive to the isotypes of USP22?

Verified Customer

Verified customer

Asked: 2019-08-08

Answer

The immunogen of M03887 anti-USP22 Monoclonal antibody is A synthesized peptide derived from human USP22 Histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. Catalyzes the deubiquitination of both histones H2A and H2B, thereby acting as a coactivator. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-08-08

Question

We are currently using anti-USP22 Monoclonal antibody M03887 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2018-12-21

Answer

The anti-USP22 Monoclonal antibody (M03887) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-12-21

Question

I was wanting to use your anti-USP22 Monoclonal antibody for IF for human uterus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human uterus identification?

T. Lewis

Verified customer

Asked: 2017-01-04

Answer

As indicated on the product datasheet, M03887 anti-USP22 Monoclonal antibody has been tested for IP, IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human uterus in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-01-04

Question

I have a question about product M03887, anti-USP22 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

A. Miller

Verified customer

Asked: 2013-08-05

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03887 anti-USP22 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2013-08-05

Order DetailsPrice
M03887

100μL

$370
M03887-30ul

30μL sample

$99
M03887-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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M03887
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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