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Product Info Summary
| SKU: | M03887 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, ICC, WB |
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Product info
Product Name
Anti-USP22 Monoclonal Antibody
SKU/Catalog Number
M03887
BM5391 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-USP22 Monoclonal Antibody catalog # M03887. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-USP22 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03887)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ADCG-21
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human USP22 Histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. Catalyzes the deubiquitination of both histones H2A and H2B, thereby acting as a coactivator.
Reactive Species
M03887 is reactive to USP22 in Human
Observed Molecular Weight
60 kDa
Calculated molecular weight
60.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03887 is guaranteed for Flow Cytometry, IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Positive Control
WB: HeLa cell
Validation Images & Assay Conditions
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Western blot analysis of USP22 expression in HeLa cell lysate.
Specific Publications For Anti-USP22 Monoclonal Antibody (M03887)
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Customer Q&As
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4 Customer Q&As for Anti-USP22 Monoclonal Antibody
Question
Is this M03887 anti-USP22 Monoclonal antibody reactive to the isotypes of USP22?
Verified Customer
Verified customer
Asked: 2019-08-08
Answer
The immunogen of M03887 anti-USP22 Monoclonal antibody is A synthesized peptide derived from human USP22 Histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. Catalyzes the deubiquitination of both histones H2A and H2B, thereby acting as a coactivator. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-08
Question
We are currently using anti-USP22 Monoclonal antibody M03887 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2018-12-21
Answer
The anti-USP22 Monoclonal antibody (M03887) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-12-21
Question
I was wanting to use your anti-USP22 Monoclonal antibody for IF for human uterus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human uterus identification?
T. Lewis
Verified customer
Asked: 2017-01-04
Answer
As indicated on the product datasheet, M03887 anti-USP22 Monoclonal antibody has been tested for IP, IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human uterus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-01-04
Question
I have a question about product M03887, anti-USP22 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Miller
Verified customer
Asked: 2013-08-05
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03887 anti-USP22 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-08-05
