Anti-USP25 Antibody

Product Name

Anti-USP25 Antibody

View all USP25 Antibodies

SKU/Catalog Number

A06182

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-USP25 Antibody (Catalog # A06182). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

USP25 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-USP25 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06182)

Host

Rabbit

Contents

USP25 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

Clone: SK7

Isotype

IgG

Immunogen

USP25 antibody was raised against a 17 amino acid peptide near the center of human USP25. The immunogen is located within amino acids 470 - 520 of USP25.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

USP25 antibody is human and mouse reactive. Multiple isoforms of USP25 are known to exist.

Reactive Species

A06182 is reactive to USP25 in Human, Mouse

Applications

A06182 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

122.218kDa

Background of USP25

USP25 (ubiquitin specific peptidase 25), also known as USP21, belongs to the peptidase C19 family and is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response (1). It contains one UBA-like domain and two UIM (ubiquitin-interacting motif) repeats. Due to alternative splicing events, USP25 is expressed as two short, ubiquitously expressed isoforms and one long, muscle-specific isoform (2). The long isoform of USP25 (USP25m) is upregulated in myogenesis and is implicated in regulation of muscular differentiation and function. USP25 is a deubiquitinating enzyme (DUB) that negatively regulates IL-17-triggered signaling (3,4).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

USP25 antibody can be used for detection of USP25 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of USP25 in mouse brain tissue lysate with USP25 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.

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Immunohistochemistry of USP25 in human brain tissue with USP25 antibody at 5 μg/mL.

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Immunofluorescence of USP25 in human brain tissue with USP25 antibody at 20 μg/mL.

Gene/Protein Information For USP25 (Source: Uniprot.org, NCBI)

Alternative Names

Deubiquitinating enzyme 25; EC 3.1.2.15; EC 3.4.19.12; ubiquitin carboxyl-terminal hydrolase 25; ubiquitin specific peptidase 25; ubiquitin specific protease USP2510USP21ubiquitin specific protease 25; ubiquitin thioesterase 25; Ubiquitin Thiolesterase 25; ubiquitin-specific processing protease 25; Ubiquitin-specific-processing protease 25; USP on chromosome 21; USP25 USP25 USP21 ubiquitin specific peptidase 25 ubiquitin carboxyl-terminal hydrolase 25|USP on chromosome 21|deubiquitinating enzyme 25|ubiquitin thioesterase 25|ubiquitin thiolesterase 25|ubiquitin-specific processing protease 25

For more info on USP25, check out the USP25 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for USP25: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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