Click image to see more details
Product Info Summary
| SKU: | M05040 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-USP28 Monoclonal Antibody
SKU/Catalog Number
M05040
BM5394 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-USP28 Monoclonal Antibody catalog # M05040. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-USP28 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05040)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ADDO-21
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human USP28 Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN.
Reactive Species
M05040 is reactive to USP28 in Human
Observed Molecular Weight
135 kDa
Calculated molecular weight
122.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05040 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
FC 1:20
Positive Control
WB: A431 cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of USP28 expression in A431 cell lysate.
Specific Publications For Anti-USP28 Monoclonal Antibody (M05040)
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Customer Q&As
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5 Customer Q&As for Anti-USP28 Monoclonal Antibody
Question
I was wanting to use your anti-USP28 Monoclonal antibody for IF for human brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain identification?
Verified Customer
Verified customer
Asked: 2020-04-20
Answer
You can see on the product datasheet, M05040 anti-USP28 Monoclonal antibody has been tested for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-20
Question
Is a blocking peptide available for product anti-USP28 Monoclonal antibody (M05040)?
Z. Krishna
Verified customer
Asked: 2020-02-18
Answer
We do provide the blocking peptide for product anti-USP28 Monoclonal antibody (M05040). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-02-18
Question
We are currently using anti-USP28 Monoclonal antibody M05040 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on goat tissues as well?
J. Brown
Verified customer
Asked: 2018-04-19
Answer
The anti-USP28 Monoclonal antibody (M05040) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-04-19
Question
Would anti-USP28 Monoclonal antibody M05040 work on canine IF with cervix carcinoma erythroleukemia?
K. Patel
Verified customer
Asked: 2018-02-19
Answer
Our lab technicians have not validated anti-USP28 Monoclonal antibody M05040 on canine. You can run a BLAST between canine and the immunogen sequence of anti-USP28 Monoclonal antibody M05040 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine cervix carcinoma erythroleukemia in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-02-19
Question
I see that the anti-USP28 Monoclonal antibody M05040 works with IF, what is the protocol used to produce the result images on the product page?
C. Patel
Verified customer
Asked: 2013-10-11
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2013-10-11
