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Product Info Summary
| SKU: | M04550 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-USP5 Antibody
SKU/Catalog Number
M04550
Size
80 µl
Description
Boster Bio Anti-USP5 Antibody (Catalog # M04550). Tested in WB application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-USP5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04550)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1340CT704.170.140
Isotype
IgG1,κ
Immunogen
This USP5 antibody is generated from a mouse immunized with a recombination protein from the human region of human USP5.
Reactive Species
M04550 is reactive to USP5 in Human, Mouse, Rat
Calculated molecular weight
95.8 kDa
Background of USP5
Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys- 63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04550 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
Validation Images & Assay Conditions
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Western blot analysis of lysates from U251, PC-3 cell line, mouse brain, rat brain and liver tissue lysates (from left to right), using USP5 Antibody. M04550 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.
Specific Publications For Anti-USP5 Antibody (M04550)
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