Anti-Utrophin/UTRN Antibody Picoband™

Product Name

Anti-Utrophin/UTRN Antibody Picoband™

View all Utrophin Antibodies

SKU/Catalog Number

A02194-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Utrophin/UTRN Antibody Picoband™ catalog # A02194-1. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Utrophin/UTRN Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02194-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Utrophin/UTRN recombinant protein (Position: L1211-K2294).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A02194-1 is reactive to UTRN in Human, Mouse

Applications

A02194-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

394 kDa

Calculated molecular weight

394.466kDa

Background of Utrophin

Utrophin is a protein that in humans is encoded by the UTRN gene. This gene shares both structural and functional similarities with the dystrophin gene. It contains an actin-binding N-terminus, a triple coiled-coil repeat central region, and a C-terminus that consists of protein-protein interaction motifs which interact with dystroglycan protein components. The protein encoded by this gene is located at the neuromuscular synapse and myotendinous junctions, where it participates in post-synaptic membrane maintenance and acetylcholine receptor clustering. Mouse studies suggest that this gene may serve as a functional substitute for the dystrophin gene and therefore, may serve as a potential therapeutic alternative to muscular dystrophy which is caused by mutations in the dystrophin gene. Alternative splicing of the utrophin gene has been described; however, the full-length nature of these variants has not yet been determined.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Human, Mouse
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Direct ELISA, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Utrophin/UTRN using anti-Utrophin/UTRN antibody (A02194-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Sw620 whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human THP-1 whole cell lysates,
Lane 5: mouse lung tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Utrophin/UTRN antigen affinity purified polyclonal antibody (Catalog # A02194-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Utrophin/UTRN at approximately 394KD. The expected band size for Utrophin/UTRN is at 394KD.

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Figure 2. IF analysis of Utrophin/UTRN using anti-Utrophin/UTRN antibody (A02194-1).
Utrophin/UTRN was detected in immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-Utrophin/UTRN Antibody (A02194-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Gene/Protein Information For UTRN (Source: Uniprot.org, NCBI)

Alternative Names

DMDLutrophin (homologous to dystrophin); DRP; DRP1DRP-1; Dystrophin-related protein 1; FLJ23678; utrophin UTRN DMDL, DRP, DRP1 utrophin utrophin|dystrophin-related protein 1

For more info on UTRN, check out the UTRN Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for UTRN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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