Product Info Summary
| SKU: | A05647 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC |
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Product info
Product Name
Anti-VAMP4 Antibody
SKU/Catalog Number
A05647
Size
100ul
Form
Liquid
Description
Boster Bio Anti-VAMP4 Antibody (Catalog# A05647). Tested in ICC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-VAMP4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05647)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human VAMP4. Immunogen sequence location: 1-50
Reactive Species
A05647 is reactive to VAMP4 in Human, Mouse
Observed Molecular Weight
72 kDa
Calculated molecular weight
16.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05647 is guaranteed for ELISA, IF, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ICC/IF 1:200 - 1:1000.
ELISA: 1:10000.
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Immunofluorescence analysis of A549 cells, using VAMP4 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-VAMP4 Antibody (A05647)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-VAMP4 Antibody
Question
We are currently using anti-VAMP4 antibody A05647 for mouse tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on canine tissues as well?
D. Williams
Verified customer
Asked: 2020-04-07
Answer
The anti-VAMP4 antibody (A05647) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-07
Question
Is a blocking peptide available for product anti-VAMP4 antibody (A05647)?
Verified Customer
Verified customer
Asked: 2019-08-06
Answer
We do provide the blocking peptide for product anti-VAMP4 antibody (A05647). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-08-06
Question
My question regards to test anti-VAMP4 antibody A05647 on mouse brodmann (1909) area 23 for research purposes, then I may be interested in using anti-VAMP4 antibody A05647 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-03-01
Answer
The products we sell, including anti-VAMP4 antibody A05647, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-03-01
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brodmann (1909) area 23 using anti-VAMP4 antibody A05647. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-11-24
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-11-24
Question
I have a question about product A05647, anti-VAMP4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Collins
Verified customer
Asked: 2016-06-22
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05647 anti-VAMP4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-06-22

