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Product Info Summary
| SKU: | A00210 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-VDR Antibody (Center)
SKU/Catalog Number
A00210
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-VDR Antibody (Center) (Catalog # A00210). Tested in WB, Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-VDR Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00210)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This VDR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 274-299 amino acids from the Central region of human VDR.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00210 is reactive to VDR in Human
Calculated molecular weight
48.3 kDa
Background of VDR
This gene encodes the nuclear hormone receptor for vitamin D3. This receptor also functions as a receptor for the secondary bile acid lithocholic acid. The receptor belongs to the family of trans-acting transcriptional regulatory factors and shows sequence similarity to the steroid and thyroid hormone receptors. Downstream targets of this nuclear hormone receptor are principally involved in mineral metabolism though the receptor regulates a variety of other metabolic pathways, such as those involved in the immune response and cancer. Mutations in this gene are associated with type II vitamin D-resistant rickets. A single nucleotide polymorphism in the initiation codon results in an alternate translation start site three codons downstream. Alternative splicing results in multiple transcript variants encoding the same protein.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00210 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
FC: 1:10-1:50
Validation Images & Assay Conditions
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Western blot analysis of lysates from PC-3, LNCaP cell line (from left to right), using VDR Antibody (Center). A00210 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody.
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VDR Antibody (Center) (Cat. #A00210) flow cytometric analysis of Hela cells (right histogram) compared to a negative control (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Specific Publications For Anti-VDR Antibody (Center) (A00210)
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