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Product Info Summary
| SKU: | A00623 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-VEGF-C Antibody
SKU/Catalog Number
A00623
Size
100μl
Form
Liquid
Description
Boster Bio Anti-VEGF-C Antibody catalog # A00623. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-VEGF-C Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00623)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human VEGFC. AA range:91-140
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00623 is reactive to VEGFC in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
46.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00623 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC: 1:100-1:300
ELISA 1:20000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of L929 cells using VEGF-C Polyclonal Antibody. Antibody was diluted at 1:2000. Secondary antibody was diluted at 1:20000
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Western blot analysis of lysate from L929 cells, using VEGFC Antibody.
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Immunohistochemical analysis of paraffin-embedded Human Liver. 1, Antibody was diluted at 1:200 (4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
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Effects of HMGB1 down-regulation on NF-κB/p65, IκBα and VEGF-C in T24 cells. A1, A2 and A3: The expression of NF-κB/p65 and VEGF-C in the shRNA group was lower than in the other two groups transfected with shNC plasmids or the untransfected controls (all P < 0.05). On the contrary, the expression of IκBα showed an opposite tendency, while no significant differences in the expression of NF-κB/p65, IκBα and VEGF-C in T24 cells were found between the CON and NC groups (all P > 0.05). The display of cropped gels is used to improve the clarity and conciseness of the presentation and all the cropped gels have been run under the same experimental conditions. : The blue areas indicate nuclei stained using 4, 6-diamidino-2-phenylindole (DAPI) and the green areas indicate the nuclear translocation of NF-κB/p65 in T24 cells transfected with shNC plasmids or untransfected and cytoplasmic localization of NF-κB/p65 in cells transfected with shRNA plasmids. The results showed that knockdown of HMGB1 expression inhibited the translocation of NF-κB/p65 from the cytoplasm to the nucleus. : EMSA revealed that the DNA-binding activity of NF-κB/p65 in T24 cells was decreased by HMGB1 knockdown.
Index in PubMed under a CC BY license. PMID: 26239046
Specific Publications For Anti-VEGF-C Antibody (A00623)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-VEGF-C Antibody
Question
Will anti-VEGF-C antibody A00623 work on dog IHC with layer of synovial tissue?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
Our lab technicians have not validated anti-VEGF-C antibody A00623 on dog. You can run a BLAST between dog and the immunogen sequence of anti-VEGF-C antibody A00623 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog layer of synovial tissue in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-08
Question
Is this A00623 anti-VEGF-C antibody reactive to the isotypes of VEGFC?
Verified Customer
Verified customer
Asked: 2019-08-07
Answer
The immunogen of A00623 anti-VEGF-C antibody is Synthesized peptide derived from the Internal region of human VEGF-C. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-07
Question
Do you have a BSA free version of anti-VEGF-C antibody A00623 available?
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-VEGF-C antibody A00623 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-17
Question
Here is the WB image, lot number and protocol we used for skin urinary bladder using anti-VEGF-C antibody A00623. Please let me know if you require anything else.
N. Yang
Verified customer
Asked: 2014-03-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-03-03
Question
We are currently using anti-VEGF-C antibody A00623 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?
D. Williams
Verified customer
Asked: 2013-03-12
Answer
The anti-VEGF-C antibody (A00623) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-03-12
