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Product Info Summary
| SKU: | M00901 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-VEGF Receptor 2 KDR Rabbit Monoclonal Antibody
SKU/Catalog Number
M00901
BM4256 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-VEGF Receptor 2 KDR Rabbit Monoclonal Antibody catalog # M00901. Tested in WB, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-VEGF Receptor 2 KDR Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00901)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
CIH-11
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human VEGF Receptor 2
Reactive Species
M00901 is reactive to KDR in Human
Observed Molecular Weight
180-250 kDa
Calculated molecular weight
151.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00901 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IP 1:20
Positive Control
WB: human placenta tissue
Validation Images & Assay Conditions
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Western blot analysis of VEGFR2 using anti-VEGFR2 antibody (M00901).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGFR2 antigen affinity purified monoclonal antibody (Catalog # M00901) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VEGFR2 at approximately 180-250 kDa. The expected band size for VEGFR2 is at 156 kDa.
Specific Publications For Anti-VEGF Receptor 2 KDR Rabbit Monoclonal Antibody (M00901)
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Customer Q&As
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15 Customer Q&As for Anti-VEGF Receptor 2 KDR Rabbit Monoclonal Antibody
Question
I was wanting to use your anti-VEGF Receptor 2 Rabbit Monoclonal antibody for IP for human umbilical vein on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human umbilical vein identification?
Verified Customer
Verified customer
Asked: 2020-03-12
Answer
It shows on the product datasheet, M00901 anti-VEGF Receptor 2 Rabbit Monoclonal antibody has been validated for IP, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human umbilical vein in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-12
Question
We are currently using anti-VEGF Receptor 2 Rabbit Monoclonal antibody M00901 for human tissue, and we are well pleased with the IP results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-17
Answer
The anti-VEGF Receptor 2 Rabbit Monoclonal antibody (M00901) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-17
Question
Is this M00901 anti-VEGF Receptor 2 Rabbit Monoclonal antibody reactive to the isotypes of KDR?
Verified Customer
Verified customer
Asked: 2019-12-27
Answer
The immunogen of M00901 anti-VEGF Receptor 2 Rabbit Monoclonal antibody is A synthesized peptide derived from human VEGF Receptor 2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-27
Question
We bought anti-VEGF Receptor 2 Rabbit Monoclonal antibody for IP on umbilical vein in the past. I am using human, and We want to use the antibody for WB next. I would like examining umbilical vein as well as lung in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-12-20
Answer
I have checked the website and datasheets of our anti-VEGF Receptor 2 Rabbit Monoclonal antibody and it seems that M00901 has been tested on human in both IP and WB. Thus M00901 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-12-20
Question
We have seen staining in human umbilical vein. Any tips? Is anti-VEGF Receptor 2 Rabbit Monoclonal antibody supposed to stain umbilical vein positively?
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
According to literature umbilical vein does express KDR. According to Uniprot.org, KDR is expressed in lung, umbilical vein, among other tissues. Regarding which tissues have KDR expression, here are a few articles citing expression in various tissues:
Umbilical vein, Pubmed ID: 15815621, 1656371, 7559454
Boster Scientific Support
Answered: 2019-11-22
Question
I am looking for to test anti-VEGF Receptor 2 Rabbit Monoclonal antibody M00901 on human umbilical vein for research purposes, then I may be interested in using anti-VEGF Receptor 2 Rabbit Monoclonal antibody M00901 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-21
Answer
The products we sell, including anti-VEGF Receptor 2 Rabbit Monoclonal antibody M00901, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-21
Question
I have a question about product M00901, anti-VEGF Receptor 2 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-07
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00901 anti-VEGF Receptor 2 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-07
Question
Will M00901 anti-VEGF Receptor 2 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-08-17
Answer
You can see on the product datasheet, M00901 anti-VEGF Receptor 2 Rabbit Monoclonal antibody as been tested on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-08-17
Question
Is a blocking peptide available for product anti-VEGF Receptor 2 Rabbit Monoclonal antibody (M00901)?
Verified Customer
Verified customer
Asked: 2018-05-29
Answer
We do provide the blocking peptide for product anti-VEGF Receptor 2 Rabbit Monoclonal antibody (M00901). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-05-29
Question
Please see the WB image, lot number and protocol we used for umbilical vein using anti-VEGF Receptor 2 Rabbit Monoclonal antibody M00901. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-12-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-12-21
Question
Will anti-VEGF Receptor 2 Rabbit Monoclonal antibody M00901 work for IP with umbilical vein?
Verified Customer
Verified customer
Asked: 2017-11-03
Answer
According to the expression profile of umbilical vein, KDR is highly expressed in umbilical vein. So, it is likely that anti-VEGF Receptor 2 Rabbit Monoclonal antibody M00901 will work for IP with umbilical vein.
Boster Scientific Support
Answered: 2017-11-03
Question
I see that the anti-VEGF Receptor 2 Rabbit Monoclonal antibody M00901 works with IP, what is the protocol used to produce the result images on the product page?
T. Zhao
Verified customer
Asked: 2017-07-31
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2017-07-31
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for umbilical vein using anti-VEGF Receptor 2 Rabbit Monoclonal antibody M00901. Let me know if you need anything else.
S. Roberts
Verified customer
Asked: 2017-07-05
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-05
Question
My colleagues were happy with the WB result of your anti-VEGF Receptor 2 Rabbit Monoclonal antibody. However we have observed positive staining in umbilical vein isoform 1: cell membrane using this antibody. Is that expected? Could you tell me where is KDR supposed to be expressed?
K. Jones
Verified customer
Asked: 2016-04-11
Answer
From what I have seen in literature, umbilical vein does express KDR. Generally KDR expresses in cell junction. endoplasmic, isoform 1: cell membrane. Regarding which tissues have KDR expression, here are a few articles citing expression in various tissues:
Umbilical vein, Pubmed ID: 15815621, 1656371, 7559454
Boster Scientific Support
Answered: 2016-04-11
Question
Is there a BSA free version of anti-VEGF Receptor 2 Rabbit Monoclonal antibody M00901 available?
D. Li
Verified customer
Asked: 2014-03-04
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-VEGF Receptor 2 Rabbit Monoclonal antibody M00901 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-03-04
