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Product Info Summary
| SKU: | M00623 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-VEGFC Antibody
SKU/Catalog Number
M00623
Size
50 µl
Description
Boster Bio Anti-VEGFC Antibody (Catalog # M00623). Tested in WB application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-VEGFC Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00623)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
197CT7.3.4
Isotype
IgG1,K
Immunogen
This VEGFC monoclonal antibody is generated from mouse immunized with VEGFC recombinant protein.
Reactive Species
M00623 is reactive to VEGFC in Human, Mouse, Rat
Calculated molecular weight
46.9 kDa
Background of VEGFC
The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family, is active in angiogenesis and endothelial cell growth, and can also affect the permeability of blood vessels. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-3 receptors. Only the fully processed form can bind and activate VEGFR-2 receptors. This protein is structurally and functionally similar to vascular endothelial growth factor D. [provided by RefSeq].
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00623 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
Validation Images & Assay Conditions
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All lanes : Anti-VEGFC at 1:2000 dilution
Lane 1: CCRF-CEM whole cell lysate
Lane 2: 293 whole cell lysate
Lane 3: HepG2 whole cell lysate
Lane 4: mouse heart lysate
Lane 5: rat heart lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 47 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Western blot analysis of lysates from 293, CEM cell line and mouse heart, rat heart tissue lysates (from left to right), using VEGFC Antibody. M00623 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.
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Western blot analysis of VEGFC using anti-VEGFC antibody (M00623).
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: LPS treated human retinal microvascular endothelial cells lysates,
Lane 2: Dexamethasone treated human retinal microvascular endothelial cells lysates,
Lane 3: Medicine treated human retinal microvascular endothelial cells lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGFC antigen affinity purified polyclonal antibody (M00623) at 1:2000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054) at a dilution of 1:10000 for 1 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with ChemiDoc MP system.
Specific Publications For Anti-VEGFC Antibody (M00623)
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Customer Reviews
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1 Reviews For Anti-VEGFC Antibody
WB results showed that VEGFC (M00623) was upregulated by LPS and reduced after dexamethasone or homemade drug treatment, with clear and specific bands, indicating an anti-inflammatory effect.
Excellent
| SKU | M00623 |
|---|---|
| Application | Western Blot |
| Sample | Human retinal microvascular endothelial cells |
| Sample Processing Description | Human retinal microvascular endothelial cells treated with LPS and then with dexamethasone or homemade drug. |
| Other Reagents | RIPA lysis buffer, Protease inhibitor, Running buffer, Transfer buffer, Blocking buffer |
| Primary Antibody | VEGFC Antibody |
| Primary Incubation | 1:2000, overnight at 4 ℃ |
| Secondary Antibody | HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054) |
| Secondary Incubation | 1:10000, 1 h in RT |
| Detection | Substrate: ECL substrate, Image system:ChemiDoc MP |
| Results Summary | VEGFC is an important signaling protein in vivo that primarily acts on lymphatic and blood vascular endothelial cells, promoting lymphangiogenesis and angiogenesis. WB results showed that VEGFC expression was increased in LPS-stimulated human retinal microvascular endothelial cells and decreased after treatment with dexamethasone or a homemade drug, indicating the anti-inflammatory effect of the homemade drug. |
Kaitong Yang, Jiangnan University, Wuxi Medical College
Verified customer
Submitted 2026-03-04
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