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Product Info Summary
| SKU: | A09587 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Vezatin VEZT Antibody
SKU/Catalog Number
A09587
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Vezatin VEZT Antibody (Catalog # A09587). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
VEZT antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Vezatin VEZT Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09587)
Host
Rabbit
Contents
VEZT antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
VEZT antibody was raised against a 17 amino acid peptide near the center of human VEZT. The immunogen is located within amino acids 480 - 530 of VEZT.
Cross-reactivity
VEZT antibody is human, mouse and rat reactive. Multiple isoforms are known to exist.
Reactive Species
A09587 is reactive to VEZT in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
88.7 kDa
Background of VEZT
Vezatin (VEZT) is a transmembrane protein which localizes to adherens junctions and binds to myosin VIIA and the cadherin-catenins complex (1). Expression of this gene in gastric cancer tissues is decreased due to hypermethylation of the promoter. Expression of this gene may also be inhibited by binding of a specific microRNA to a target sequence in the 3' UTR of the transcripts (2). VEZT has also been shown to be required for morphogenesis of the preimplantation mouse embryo (3).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09587 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
VEZT antibody can be used for detection of VEZT by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of VEZT in 293 cell lysate with VEZT antibody at (A) 1 and (B) 2 μg/ml.
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Immunohistochemistry of VEZT in rat kidney tissue with VEZT antibody at 5 μg/ml.
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Immunofluorescence of TRIP12 in rat colon tissue with TRIP12 antibody at 20 μg/ml.
Specific Publications For Anti-Vezatin VEZT Antibody (A09587)
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