Anti-Vimentin Antibody (Monoclonal, 35V46)

Product Name

Anti-Vimentin Antibody (Monoclonal, 35V46)

View all Vim Antibodies

SKU/Catalog Number

M00235-7

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Vimentin Antibody (Monoclonal, 35V46) catalog # M00235-7. Tested in IF, IHC, WB applications. This antibody reacts with Human, Pig, Rabbit, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Vimentin Antibody (Monoclonal, 35V46) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00235-7)

Host

Mouse

Contents

Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

35V46

Isotype

Mouse IgG1

Immunogen

Pig eye lens vimentin.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

M00235-7 is reactive to Vim in Human, Pig, Rabbit, Rat

Calculated molecular weight

53.7 kDa

Background of Vim

The VIM gene was one of many that Gieser and Swaroop (1992) recovered from a subtracted cDNA library for retinal pigment epithelium. Vimentin gene express in human lymphocytes and in Burkitt's lymphoma cells. Vimentin is secreted by activated macrophages. The gene encoding human vimentin is located on the short arm of chromosome 10.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00235-7 is guaranteed for IF, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.5-1μg/ml, Human, rabbit, pig, rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, rabbit, pig, rat
Immunofluorescence, 5μg/ml, Human

Validation Images & Assay Conditions

There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.

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