Vimentin Antibodies

AND VIM ELISA kits, Vimentin Proteins

Many biological assays use antibodies to detect Vimentin, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with Vimentin in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop Vimentin antibodies...

We validate the specificity of these antibodies to Vimentin by testing them on tissues known to express VIM positively and negatively. Browse below to find the VIM antibody that suites your experiment. We have 14 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Designing a Western blot for Vimentin? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.

Open the VIM Western Blot Guide

VIM Infographic by Boster Bio

All VIM Products

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Product

Figures

Specificity

Applications

Price

Anti-Vimentin Antibody Picoband®

88 Citations

IHC analysis of Vimentin using anti-Vimentin antibody (PB9359)

+20

Human, Mouse, Rat

IF, IHC, WB

$370
Cat # PB9359
100 μg/vial

Anti-Vimentin Rabbit Monoclonal Antibody

41 Citations

Western blot analysis of Vimentin using anti-Vimentin antibody (M00235-1)

IHC analysis of Vimentin using anti-Vimentin antibody (M00235-1)

+25

Human, Mouse, Rat

Flow Cytometry, IF, IHC, ICC, WB

$370
Cat # M00235-1
100 μl

Anti-Phospho-Vimentin (S39) Rabbit Monoclonal Antibody

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature

Western blot analysis of Phospho-Vimentin (S39) expression in HeLa treated with Calyculin A cell lysate

Human, Mouse, Rat

$370
Cat # M00235S39
100 µl/vial

Anti-Vimentin Antibody

All lanes : Anti-VIME Antibody at 1:2000 dilutionLane 1: Hela whole cell lysateLane 2: 293 whole cell lysateLane 3: U-2OS whole cell lysateLane 4: Jurkat whole cell lysateLane 5: K562 whole cell lysateLane 6: NIH/3T3 whole cell lysateLane 7: C2C12 whole cell lysateLysates/proteins at 20 µg per lane

All lanes : Anti-VIME Antibody at 1:4000 dilutionLane 1: Hela whole cell lysateLane 2: 293 whole cell lysateLane 3: U-2OS whole cell lysateLane 4: Jurkat whole cell lysateLane 5: K562 whole cell lysateLane 6: NIH/3T3 whole cell lysateLane 7: C2C12 whole cell lysateLysates/proteins at 20 µg per lane

Confocal immunofluorescent analysis of Vimentin Antibody with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green)

Human, Mouse

Flow Cytometry, IF, WB, IHC-P-Leica

$299
Cat # A00235-1
80 µl

Anti-Vimentin Rabbit Monoclonal Antibody

Human colon was stained with anti-Vimentin rabbit antibody

Mouse colon was stained with anti-Vimentin rabbit antibody

Rat colon was stained with anti-Vimentin rabbit antibody

Human, Mouse, Rat

ELISA, IP, IF, IHC, WB

$146
Cat # M00235-2
20μL, 40μL, 100μL, 200μL

Anti-Vimentin (Phospho-Tyr38) Antibody

Western Blot analysis of HELA cells using Antibody diluted at 500

Western blot analysis of lysates from 293 cells treated with paclitaxel, using p-Vimentin (Phospho-Ser38) antibody

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Vimentin (Phospho-Ser38) Antibody

Human, Mouse, Rat

ELISA, WB

$380
Cat # A00235Y38
100ul

Vimentin (Phospho-Ser56) Colorimetric Cell-Based ELISA Kit

Western blot analysis of extracts from A549 cells treated with Nocodazole 1ug/ml 16h

Human

ELISA

$540
Cat # EKC2343
1 kit, containing two 96-well plates and all necessary reagents

Vimentin (Phospho-Ser83) Colorimetric Cell-Based ELISA Kit

Western blot analysis of extracts from 293 cells treated with paclitaxel 1uM 24h

Human, Mouse, Rat

ELISA

$540
Cat # EKC2342
1 kit, containing two 96-well plates and all necessary reagents

Vimentin Colorimetric Cell-Based ELISA Kit

Western blot analysis of extracts from A549 cells

Human, Mouse, Rat

ELISA

$480
Cat # EKC1595
1 kit, containing one 96-well plate and all necessary reagents

AIRE (Phospho-Ser156) Colorimetric Cell-Based ELISA Kit

Western blot analysis of extracts from HeLa cells treated with Hu 2nM 24h

Human

ELISA

$540
Cat # EKC2603
1 kit, containing two 96-well plates and all necessary reagents

Vimentin (VIM) (NM_003380) Human Over-expression Lysate

Human

$628
Cat # LS007431
20 µg, 100 µg

Anti-VMAC Antibody

Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°over night

Human, Mouse, Rat

ELISA, WB

$380
Cat # A18238
100ul

Anti-Zebrafish Vimentin/VIM Antibody Picoband®

Western blot analysis of Vimentin/VIM using anti-Vimentin/VIM antibody (AZQ9YHX5)

IHC analysis of Vimentin/VIM using anti-Vimentin/VIM antibody (AZQ9YHX5)

Zebrafish

IHC, WB

$470
Cat # AZQ9YHX5
100 μg/vial

Anti-Vimentin Antibody (Monoclonal, 35V46)

Human, Pig, Rabbit, Rat

IF, IHC, WB

$370
Cat # M00235-7
100 μg/vial

VIM Overview

Facts about Vimentin.

VIM 3D structure. Source: alphafold

Vimentin (VIM)

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is connected to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.

Gene Information

Human
Gene Name:	VIM
Uniprot:	P08670
Entrez:	7431

Family

Belongs to:

intermediate filament family

Alternative Names

FLJ36605; VIM; Vimentin

Molecular Weight

Mass (kDA):
53.652 kDA

Genomic Context


Human

Location:	10p13
Sequence:	10; NC_000010.11 (17228241..17237593)

Tissue Specificity

Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines.

Subcellular Localizations

Cytoplasm. Cytoplasm, cytoskeleton. Nucleus matrix.

Diseases

Parkinson Disease
Neoplasms
Essential Tremor
Malignant Neoplasms
Carcinoma
Movement Disorders
Pneumonia
Pseudomonas Infections
Globus Hystericus
Dyskinetic Syndrome
Infective Disorder
Multiple Acyl Coenzyme A Dehydrogenase Deficiency

Dystonia Disorders
Neoplasm Metastasis
Malignant Paraganglionic Neoplasm
Tremor, Unspecified
Cross Infection
Cell Invasion
Sclerosis

Interacting Proteins

AKT1
AKT2
BCAS3
BECN1
CRMP1

CWF19L2
DES
GFAP
KAT7
KRT20

KRT8
NCR1
NEFL
NEFM
NES

NOD2
PAWR
PKN1
PNMA5
PPL

PRPH
RAD23B
TRIM16
TUBA1C
TXLNB

YWHAZ

Pathways

Localization
Regeneration
Methylation
Cell Proliferation
Coagulation
Pathogenesis
Conjugation
Dna Methylation
Neurogenesis
Cell Adhesion
Transport
Secretion
Cell Migration

Proteolysis
Cell Cycle
Cell Differentiation
Mating
Transdifferentiation
Neuroprotection
Dedifferentiation

PTMs

Phosphorylation
Methylation
Farnesylation
Cleavage
Oxidation
Demethylation
Citrullination

Prenylation
Biotinylation
Nitration
Reduction
Deacetylation
Glycosylation
Acetylation

Research Areas

Cancer
Cell Biology
Cellular Markers
Growth and Development
Hypoxia

Neuronal Cell Markers
Neuroscience
Signal Transduction
Stem Cell Markers

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/VIM

References

PMID: 3467175 by Ferrari S., et al. Coding sequence and growth regulation of the human vimentin gene.

PMID: 2251132 by Honore B., et al. Nucleotide sequence of cDNA covering the complete coding part of the human vimentin gene.

126 Publications of Boster Vimentin Antibodies & ELISA Kits

PubMed ID: 10.7150/jca.49138, LINC00184 involved in the regulatory network of ANGPT2 via ceRNA mediated miR-14...
PubMed ID: 10.3892/ol.2020.12020, Mechanism of transmembrane and coiled‑coil domain 1 in the regulation of proli...
PubMed ID: 10.3892/ijo.2018.4564, Histone deacetylase HDAC4 promotes the proliferation and invasion of glioma cell...
PubMed ID: 10.3892/ijmm.2021.5071, Microrchidia family CW‑type zinc finger 2 promotes the proliferation, invasio...
PubMed ID: 10.3892/ijmm.2019.4226, Botulinum toxin type A prevents the phenotypic transformation of fibroblasts ind...
PubMed ID: 10.3892/etm.2021.9905, Negative pressure wound therapy enhances bone regeneration compared with convent...
PubMed ID: 10.3892/etm.2021.10087, Effect of Porphyromonas gingivalis lipopolysaccharide on calcification ...
PubMed ID: 10.3390/cancers12030579, Development and Radiation Response Assessment in A Novel Syngeneic Mouse Model o...
PubMed ID: 10.1186/s12906-021-03213-5, Baicalein inhibits inflammatory response and promotes osteogenic activity in per...
PubMed ID: 10.1158/1078-0432.CCR-08-0520, Identification of Metastasis-Related Proteins and Their Clinical Relevance to Tr...
PubMed ID: 10.1155/2021/8868700, Elevated Circular RNA PVT1 Promotes Eutopic Endometrial Cell Proliferation and I...
PubMed ID: 10.1007/s13770-021-00405-5, The Therapeutic Effects of Exosomes Derived from Human Umbilical Cord Mesenchyma...
PubMed ID: , A synthetic cantharidin analog for the enhancement of doxorubicin suppression of...
PubMed ID: , The effects of human platelet lysate on dental pulp stem cells derived from impa...
PubMed ID: , Enhancement of endothelial differentiation of adipose derived mesenchymal stem c...
PubMed ID: , Perineurium-like sheath derived from long-term surviving mesenchymal stem cells ...
PubMed ID: 32194806, Fan R,Wang H,Zhang L,Ma T,Tian Y,Li H.Nanocrystallized Oleanolic Acid Better Inh...
PubMed ID: 33656053, Liu S,Xin W,Lu Q,Tang X,Wang F,Shao W,Zhang Y,Qiu J,Hua K. Knockdown of lncRNA H...
PubMed ID: -, Xu,R.,Zhou,X., Li,Y.,Ren,L.,& He,X. (2021).MicroRNA‑181a promotes epithelial�...
PubMed ID: -, Xian-Song Wang,Li Xie,Kaiyu Zheng et al.Human Peripheral Blood-derived Mast Cell...
PubMed ID: 32117780, Zhao C,She X,Zhang Y,Liu C,Li P,Chen S,Sai B,Li Y,Feng J,Liu J,Sun Y,Xiao S,Li L...

*Showing only the more recent 20. More publications can be found for each product on its corresponding product page