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Product Info Summary
| SKU: | A30448 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Vinculin VCL Antibody
SKU/Catalog Number
A30448
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Vinculin VCL Antibody (Catalog# A30448). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Vinculin VCL Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30448)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human Vinculin. Immunogen sequence location: 786-835
Reactive Species
A30448 is reactive to VCL in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
123.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30448 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC: 1:100-300
ELISA: 1:20000.
ICC/IF 1:100-300
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western Blot analysis of various cells using Vinculin Polyclonal Antibody. Secondary antibody was diluted at 1:20000
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Vinculin Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from HeLa cells, treated with forskolin 40nM 30', using Vinculin Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-Vinculin VCL Antibody (A30448)
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Vinculin VCL Antibody
Question
I see that the anti-Vinculin antibody A30448 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-24
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-03-24
Question
Is this A30448 anti-Vinculin antibody reactive to the isotypes of VCL?
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
The immunogen of A30448 anti-Vinculin antibody is A peptide derived from human Vinculin. Immunogen sequence location: 786-835. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-28
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for platelet using anti-Vinculin antibody A30448. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-10
Question
Does anti-Vinculin antibody A30448 work for WB with platelet?
Verified Customer
Verified customer
Asked: 2019-12-02
Answer
According to the expression profile of platelet, VCL is highly expressed in platelet. So, it is likely that anti-Vinculin antibody A30448 will work for WB with platelet.
Boster Scientific Support
Answered: 2019-12-02
Question
Would A30448 anti-Vinculin antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-29
Answer
As indicated on the product datasheet, A30448 anti-Vinculin antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-29
Question
We have seen staining in mouse uterus. Are there any suggestions? Is anti-Vinculin antibody supposed to stain uterus positively?
Verified Customer
Verified customer
Asked: 2019-08-13
Answer
Based on literature uterus does express VCL. Based on Uniprot.org, VCL is expressed in myometrium, endothelial cell, retina, prostate, fetal brain cortex, uterus, platelet, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have VCL expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Endothelial cell, Pubmed ID: 2116004
Liver, Pubmed ID: 24275569
Platelet, Pubmed ID: 12665801
Prostate, Pubmed ID: 15489334
Retina, Pubmed ID: 17974005
Uterus, Pubmed ID: 1339348
Boster Scientific Support
Answered: 2019-08-13
Question
We are currently using anti-Vinculin antibody A30448 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-11
Answer
The anti-Vinculin antibody (A30448) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-11
Question
Is a blocking peptide available for product anti-Vinculin antibody (A30448)?
Verified Customer
Verified customer
Asked: 2019-06-18
Answer
We do provide the blocking peptide for product anti-Vinculin antibody (A30448). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-06-18
Question
We bought anti-Vinculin antibody for IHC on platelet a few years ago. I am using human, and We want to use the antibody for IF next. My lab would like examining platelet as well as endothelial cell in our next experiment. Could give a recommendation on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2019-05-30
Answer
I have checked the website and datasheets of our anti-Vinculin antibody and it seems that A30448 has been validated on human in both IHC and IF. Thus A30448 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-05-30
Question
See below the WB image, lot number and protocol we used for platelet using anti-Vinculin antibody A30448. Please let me know if you require anything else.
A. Lewis
Verified customer
Asked: 2019-02-08
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-02-08
Question
Is there a BSA free version of anti-Vinculin antibody A30448 available?
Verified Customer
Verified customer
Asked: 2018-05-25
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Vinculin antibody A30448 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-05-25
Question
Our lab want to know about to test anti-Vinculin antibody A30448 on human platelet for research purposes, then I may be interested in using anti-Vinculin antibody A30448 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
E. Kulkarni
Verified customer
Asked: 2018-04-09
Answer
The products we sell, including anti-Vinculin antibody A30448, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-04-09
Question
I was wanting to use your anti-Vinculin antibody for WB for human platelet on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human platelet identification?
R. Carter
Verified customer
Asked: 2018-03-13
Answer
As indicated on the product datasheet, A30448 anti-Vinculin antibody has been tested for IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human platelet in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-03-13
Question
I have a question about product A30448, anti-Vinculin antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
W. Yang
Verified customer
Asked: 2017-12-28
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30448 anti-Vinculin antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-12-28
Question
My team were content with the WB result of your anti-Vinculin antibody. However we have seen positive staining in cervix carcinoma cell membrane using this antibody. Is that expected? Could you tell me where is VCL supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-06-15
Answer
From what I have seen in literature, cervix carcinoma does express VCL. Generally VCL expresses in cell membrane. Regarding which tissues have VCL expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Endothelial cell, Pubmed ID: 2116004
Liver, Pubmed ID: 24275569
Platelet, Pubmed ID: 12665801
Prostate, Pubmed ID: 15489334
Retina, Pubmed ID: 17974005
Uterus, Pubmed ID: 1339348
Boster Scientific Support
Answered: 2017-06-15
Question
I am interested in using your anti-Vinculin antibody for lamellipodium assembly studies. Has this antibody been tested with western blotting on breast carcinoma tissue? We would like to see some validation images before ordering.
C. Wu
Verified customer
Asked: 2016-10-21
Answer
Thanks for your inquiry. This A30448 anti-Vinculin antibody is tested on hela cells, breast carcinoma tissue, a549 cells. It is guaranteed to work for IF, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2016-10-21
