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Product Info Summary
| SKU: | M01207-4 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Chicken, Human, Monkey, Mouse, Rat |
| Host: | Mouse |
| Application: | IF, IHC-F, ICC, WB |
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Product info
Product Name
Anti-Vinculin VCL Antibody (Monoclonal, 35V45)
SKU/Catalog Number
M01207-4
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Vinculin VCL Antibody (Monoclonal, 35V45) catalog # M01207-4. Tested in IF, IHC-F, ICC, WB applications. This antibody reacts with Chicken, Human, Monkey, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Vinculin VCL Antibody (Monoclonal, 35V45) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01207-4)
Host
Mouse
Contents
Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
35V45
Isotype
Mouse IgG1
Immunogen
Human vinculin, purified from uterus.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
M01207-4 is reactive to VCL in Chicken, Human, Monkey, Mouse, Rat
Calculated molecular weight
116.6 kDa
Background of VCL
Vinculin is a cytoskeletal protein associated with the cytoplasmic face of both cell-cell and cell-extracellular matrix adherens-type junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Both human and chicken embryo sequences of vinculin contain 1,066 amino acids and, furthermore, that the 2 proteins exhibit a high level of sequence identity (greater than 95%). Vinculinis mapped to 10q22.1-q23.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01207-4 is guaranteed for IF, IHC-F, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 1-2μg/ml, Human, chicken, monkey, mouse,
rat
Immunohistochemistry (Frozen Section), 2-4μg/ml, Human, chicken, mouse, rat, -
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Validation Images & Assay Conditions
There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.
Specific Publications For Anti-Vinculin VCL Antibody (Monoclonal, 35V45) (M01207-4)
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