Anti-VISA MAVS Antibody

Product Name

Anti-VISA MAVS Antibody

View all MAVS Antibodies

SKU/Catalog Number

A00169-2

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-VISA MAVS Antibody (Catalog # A00169-2). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

VISA antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-VISA MAVS Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00169-2)

Host

Rabbit

Contents

VISA Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

VISA antibody was raised against a 13 amino acid synthetic peptide from near the amino terminus of human VISA. The immunogen is located within the first 50 amino acids of VISA.

Reactive Species

A00169-2 is reactive to MAVS in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

56.5 kDa

Background of MAVS

Two distinct signaling pathways activate the host innate immunity against viral infection. One pathway is reliant on members of the Toll-like receptor (TLR) family while the other uses the RNA helicase RIG-I as a receptor for intracellular viral double-stranded RNA as a trigger for the immune response. VISA is a mitochondrial membrane protein that was identified as a critical component in the IFN-b signaling pathways that recruits IRF-3 to RIG-I, leading to its activation and that of NF-κB. VISA is also thought to interact with other components of the innate immune pathway such as the TLR adapter protein TRIF, TRAF2 and TRAF6. VISA also interacts with the IKKα, IKKβ and IKKε kinases through its C-terminal region. Cleavage of this region by the Hepatitis C virus (HCV) protease allows HCV to escape the host immune system. At least three isoforms of VISA are known to exist.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00169-2 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

VISA antibody can be used for detection of VISA by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information MAVS

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