Product Info Summary
SKU: | A03768-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Monkey |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-VPAC2/VIPR2 Antibody Picoband™
View all VIPR2/VPAC2 Antibodies
SKU/Catalog Number
A03768-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-VPAC2/VIPR2 Antibody Picoband™ catalog # A03768-1. Tested in IHC, WB applications. This antibody reacts with Human, Monkey.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-VPAC2/VIPR2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03768-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human VPAC2/VIPR2, which shares 94.4% and 85% amino acid (aa) sequence identity with mouse and rat VIPR2, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03768-1 is reactive to VIPR2 in Human, Monkey
Applications
A03768-1 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
49 kDa
Calculated molecular weight
49.479kDa
Background of VIPR2/VPAC2
Vasoactive intestinal peptide receptor 2 also known as VPAC2, is a G-protein coupled receptor that in humans is encoded by the VIPR2 gene. This gene encodes a receptor for vasoactive intestinal peptide, a small neuropeptide. Vasoactive intestinal peptide is involved in smooth muscle relaxation, exocrine and endocrine secretion, and water and ion flux in lung and intestinal epithelia. Its actions are effected through integral membrane receptors associated with a guanine nucleotide binding protein which activates adenylate cyclase.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Monkey
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of VPAC2/VIPR2 using anti-VPAC2/VIPR2 antibody (A03768-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: monkey COS-7 whole cell lysates,
Lane 2: human MOLT-4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VPAC2/VIPR2 antigen affinity purified polyclonal antibody (Catalog # A03768-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VPAC2/VIPR2 at approximately 49 kDa. The expected band size for VPAC2/VIPR2 is at 49 kDa.
Click image to see more details
Figure 2. IHC analysis of VPAC2/VIPR2 using anti-VPAC2/VIPR2 antibody (A03768-1).
VPAC2/VIPR2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VPAC2/VIPR2 Antibody (A03768-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of VPAC2/VIPR2 using anti-VPAC2/VIPR2 antibody (A03768-1).
VPAC2/VIPR2 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VPAC2/VIPR2 Antibody (A03768-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For VIPR2 (Source: Uniprot.org, NCBI)
Gene Name
VIPR2
Full Name
Vasoactive intestinal polypeptide receptor 2
Weight
49.479kDa
Superfamily
G-protein coupled receptor 2 family
Alternative Names
FLJ16511; Helodermin-preferring VIP receptor; PACAP type III receptor; PACAPR3; PACAP-R3; PACAP-R-3; Pituitary adenylate cyclase-activating polypeptide type III receptor; vasoactive intestinal peptide receptor 2; vasoactive intestinal polypeptide receptor 2; VIP R2; VIP2R; VIPR2; VIP-R-2; VPAC2; VPCAP2R VIPR2 C16DUPq36.3, DUP7q36.3, PACAP-R-3, PACAP-R3, VIP-R-2, VPAC2, VPAC2R, VPCAP2R vasoactive intestinal peptide receptor 2 vasoactive intestinal polypeptide receptor 2|PACAP type III receptor|VIP and PACAP receptor 2|helodermin-preferring VIP receptor|pituitary adenylate cyclase-activating polypeptide type III receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on VIPR2, check out the VIPR2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VIPR2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-VPAC2/VIPR2 Antibody Picoband™ (A03768-1)
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No publications found for A03768-1
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