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Product Info Summary
| SKU: | A04941 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-WAC Antibody
SKU/Catalog Number
A04941
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-WAC Antibody (Catalog # A04941). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
WAC antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-WAC Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04941)
Host
Rabbit
Contents
WAC Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
WAC antibody was raised against a 19 amino acid peptide near the center of human WAC . The immunogen is located within amino acids 210 - 260 of WAC.
Cross-reactivity
At least two isoforms of WAC are known to exist; this antibody will detect the larger isoform.
Reactive Species
A04941 is reactive to WAC in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
70.7 kDa
Background of WAC
The WW domain containing adaptor with coiled-coil protein (WAC) contains a WW domain that mediates protein-protein interactions and colocalizes with RNA splicing factor SC35. Further studies have indicated that WAC is a functional partner of the RNF20/40 complex that ubiquitinates Histone H2B, and that WAC regulates H2B ubiquitination. WAC targets RNF20/40 to associate with RNA polymerase II complex for H2B ubiquitination at active transcription sites. WAC-dependent transcription is also important for cell-cycle checkpoint activation in response to genotoxic stress.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04941 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WAC Antibody can be used for detection of WAC by Western blot at 1 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of WAC in human testis tissue lysate with WAC antibody at 1 μg/mL.
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Immunohistochemistry of WAC in mouse testis tissue with WAC antibody at 2.5 μg/ml.
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Immunofluorescence of WAC in mouse testis tissue with WAC antibody at 5 μg/ml.
Specific Publications For Anti-WAC Antibody (A04941)
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