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Product Info Summary
| SKU: | A13595 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-WDR18 Antibody
SKU/Catalog Number
A13595
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-WDR18 Antibody (Catalog # A13595). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
WDR18 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-WDR18 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13595)
Host
Rabbit
Contents
WDR18 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
WDR18 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human WDR18. The immunogen is located within the last 50 amino acids of WDR18.
Cross-reactivity
WDR18 antibody is predicted to not cross-react with other WDR family members.
Reactive Species
A13595 is reactive to WDR18 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
47.4 kDa
Background of WDR18
WD repeat domain 18 (WDR18) is a member of the WD repeat protein family, which is involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. WD repeats are minimally conserved regions of approximately 40 amino acids that commonly form a tertiary propeller structure. WDR18 is a 432 amino acid protein that contains six WD repeats and is encoded by a gene located on human chromosome 19. WDR18 may play a role during development. The complex PELP1-TEX10-WDR18 is required for the maturation and nucleolar release of the large ribosomal subunit.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13595 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WDR18 antibody can be used for detection of WDR18 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in rat samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of WDR18 in rat lung tissue lysate with WDR18 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of WDR18 in human lung tissue with WDR18 antibody at 2.5 μg/mL.
Specific Publications For Anti-WDR18 Antibody (A13595)
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