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Product Info Summary
| SKU: | M00199-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Wilms Tumor Protein WT1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00199-1
BM4216 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Wilms Tumor Protein WT1 Rabbit Monoclonal Antibody catalog # M00199-1. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Wilms Tumor Protein WT1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00199-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
CEH-23
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Wilms Tumor Protein
Reactive Species
M00199-1 is reactive to WT1 in Human, Mouse
Observed Molecular Weight
55 kDa
Calculated molecular weight
49.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00199-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:20
Positive Control
IHC: mouse kidney tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded mouse kidney, using Wilms Tumor Protein Antibody.
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Construction of db/db model to evaluate changes in renal pathology lipid metabolism. Two groups of twenty-week-old mice were analyzed: db/m (n=6) and db/db (n=6). A-B. Representative PAS staining of glomeruli in each group, and the semiquantitative analysis of mesangial area ratio. (**<0.01, scale bar: 50 μm). C-G. Biochemical examination: (C) UACR, (D) serum creatinine, (E) blood glucose, (F) serum cholesterol, (G) serum triglycerides. (**<0.01). H. Representative transmission electron microscopy (TEM) images of podocyte foot processes and glomerular basement membrane (GBM) in each group. (**<0.01, scale bar: 10 μm). I-J. Representative immunofluorescence double staining of BODIPY and WT1 in the glomeruli of db/m and db/db mice, and semiquantitative analysis. (**<0.01, scale bar:50μm). K-L. Oil Red O staining of lipid droplets (LDs) in the glomeruli of db/m and db/db mice, and semiquantitative analysis. (**<0.01, scale bar: 40 μm).
Index in PubMed under a CC BY license. PMID: 40607256
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of WT1 expression in K562 cell lysate.
Specific Publications For Anti-Wilms Tumor Protein WT1 Rabbit Monoclonal Antibody (M00199-1)
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Customer Q&As
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7 Customer Q&As for Anti-Wilms Tumor Protein WT1 Rabbit Monoclonal Antibody
Question
I was wanting to use your anti-Wilms Tumor Protein Rabbit Monoclonal antibody for IF for human placenta on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human placenta identification?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
It shows on the product datasheet, M00199-1 anti-Wilms Tumor Protein Rabbit Monoclonal antibody has been validated for IF, IHC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-22
Question
I have a question about product M00199-1, anti-Wilms Tumor Protein Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-11
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00199-1 anti-Wilms Tumor Protein Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-11
Question
Will anti-Wilms Tumor Protein Rabbit Monoclonal antibody M00199-1 work on feline IHC with placenta?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
Our lab technicians have not validated anti-Wilms Tumor Protein Rabbit Monoclonal antibody M00199-1 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Wilms Tumor Protein Rabbit Monoclonal antibody M00199-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline placenta in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-16
Question
I see that the anti-Wilms Tumor Protein Rabbit Monoclonal antibody M00199-1 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-06-26
Question
I have attached the WB image, lot number and protocol we used for placenta using anti-Wilms Tumor Protein Rabbit Monoclonal antibody M00199-1. Please let me know if you require anything else.
N. Singh
Verified customer
Asked: 2019-05-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-20
Question
Is a blocking peptide available for product anti-Wilms Tumor Protein Rabbit Monoclonal antibody (M00199-1)?
Verified Customer
Verified customer
Asked: 2018-06-13
Answer
We do provide the blocking peptide for product anti-Wilms Tumor Protein Rabbit Monoclonal antibody (M00199-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-06-13
Question
We are currently using anti-Wilms Tumor Protein Rabbit Monoclonal antibody M00199-1 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2018-04-19
Answer
The anti-Wilms Tumor Protein Rabbit Monoclonal antibody (M00199-1) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-04-19
