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- Table of Contents
WT1 antibody (M00199-1) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 8 Publications
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Immunohistochemical analysis of paraffin-embedded mouse kidney, using Wilms Tumor Protein Antibody.
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Anti-Wilms Tumor Protein WT1 Rabbit Monoclonal Antibody
WT1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse | Rabbit | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
WT1 antibody was positively detected by IHC in:
mouse kidney tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:20
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
ICC
IF
FC
Mouse
Recommend
Tested
Recommend
Recommend
Recommend
Human
Recommend
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-Wilms Tumor Protein WT1 Rabbit Monoclonal Antibody
View all WT1 Antibodies
View all WT1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
CEH-23
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Wilms Tumor Protein
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
55 kDa
Calculated Molecular Weight
49.2 kDa
Cite This Product
Anti-Wilms Tumor Protein WT1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00199-1)
Protocols
Immunohistochemistry (IHC) protocol for WT1 antibody
Publications
Application:
Species:
PLIN5 Protects Against Ang II-Induced Podocyte Lipotoxicity by Interacting With FKBP8 and Preserving Lipid Droplet–Mitochondria Contact
Published: 2026-06-30
Journal: CELL PROLIFERATION
Application: IF
Impact Factor: 7.60
Species: Mouse
PMID: 42379659
Identification of Common Genes Regulated by ER Stress During the Development of Diabetic Nephropathy Based on Human Transcriptome Datasets and an In Vivo Mouse Model
Published: 2026-03-08
Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Impact Factor: 4.90
PMID: 41828705
Impaired glycolysis-derived serine metabolism as a key driver of podocyte injury with senescence
Published: 2025-12-04
Journal: Nature Communications
Application: IF
Impact Factor: 15.70
Species: Mouse
PMID: 41345115
APT1-derived depalmitoylation of CD36 alleviates diabetes-induced lipotoxicity in podocytes
Published: 2025-06-09
Journal: International Journal of Biological Sciences
Application: IF
Impact Factor: 10.00
Species: Mouse
PMID: 40607256
Integrating collecting systems in human kidney organoids through fusion of distal nephron to ureteric bud
Published: 2025-05-08
Journal: Cell Stem Cell
Application: IF
Impact Factor: 20.40
Species: Human
PMID: 40345193
Geniposide Improves Diabetic Nephropathy by Enhancing ULK1-Mediated Autophagy and Reducing Oxidative Stress through AMPK Activation
Published: 2021-02-06
Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Application: IHC
Impact Factor: 5.92
Species: Mouse
PMID: 33562139
P2Y2R contributes to the development of diabetic nephropathy by inhibiting autophagy response
Published: 2020-09-25
Journal: Molecular Metabolism
Application: IHC
Impact Factor: 6.45
Species: Mouse
PMID: 32987187
Supplementation of Abelmoschus manihot Ameliorates Diabetic Nephropathy and Hepatic Steatosis by Activating Autophagy in Mice
Published: 2018-11-07
Journal: Nutrients
Application: IHC
Impact Factor: 4.20
Species: Mouse
PMID: 30405076
Review
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Product Promise
We promise Anti-Wilms Tumor Protein WT1 Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00199-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 1, 2026