Anti-Wilms Tumor Protein WT1 Rabbit Monoclonal Antibody

Boster Bio Anti-Wilms Tumor Protein WT1 Rabbit Monoclonal Antibody catalog # M00199-1. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse. Cited in 3 publication(s).

Product Info Summary

SKU: M00199-1
Size: 100 μl
Reactive Species: Human, Mouse
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-Wilms Tumor Protein WT1 Rabbit Monoclonal Antibody

See all WT1 products

SKU/Catalog Number

M00199-1

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Wilms Tumor Protein WT1 Rabbit Monoclonal Antibody catalog # M00199-1. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Wilms Tumor Protein WT1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00199-1)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

CEH-23

Immunogen

A synthesized peptide derived from human Wilms Tumor Protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00199-1 is reactive to WT1 in Human, Mouse

Applications

M00199-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB 1:500-1:1000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Flow Cytometry 1:50

Validation Images & Assay Conditions

Gene/Protein Information For WT1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P19544

Gene ID

7490

Gene Name

WT1

Full Name

Wilms tumor protein

Weight

49.188kDa

Superfamily

EGR C2H2-type zinc-finger protein family

Alternative Names

AWT1; GUD; NPHS4GUD; WAGR; Wilms tumor 1; Wilms tumor protein; WIT-2; WT1; WT33 WT1 AWT1, GUD, NPHS4, WAGR, WIT-2, WT33 WT1 transcription factor Wilms tumor protein|Wilms tumor 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on WT1, check out the WT1 Infographic

WT1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for WT1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

M00199-1 has been cited in 3 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Dusabimana T,Kim SR,Park EJ,Je J,Jeong K,Yun SP,Kim HJ,Kim H,Park SW.P2Y2R contributes to the development of diabetic nephropathy by inhibiting autophagy response.Mol Metab.2020 Dec;42:101089.doi:10.1016/j.molmet.2020.101089.Epub 2020 Sep 25.PMID:32987187;PMCID:PMC7568185.
Species: Mouse
M00199-1 usage in article: APP:IHC, SAMPLE:KIDNEY TISSUE, DILUTION:NA

Dusabimana T,Park EJ,Je J,Jeong K,Yun SP,Kim HJ,Kim H,Park SW.Geniposide Improves Diabetic Nephropathy by Enhancing ULK1-Mediated Autophagy and Reducing Oxidative Stress through AMPK Activation.Int J Mol Sci.2021 Feb 6;22(4):1651.doi:10.3390/ijms22041651.
Species: Mouse
M00199-1 usage in article: APP:IHC, SAMPLE:KIDNEY TISSUE, DILUTION:NA

Sun D, Bu L, Liu C, Yin Z, Zhou X, Li X, Xiao A. Plos One. 2013 May 28;8(5):E65042. Doi: 10.1371/Journal.Pone.0065042. Print 2013. Therapeutic Effects Of Human Amniotic Fluid-Derived Stem Cells On Renal Interstitial Fibrosis In A Murine Model Of U...

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7 Customer Q&As for Anti-Wilms Tumor Protein WT1 Rabbit Monoclonal Antibody

Question

I was wanting to use your anti-Wilms Tumor Protein Rabbit Monoclonal antibody for IF for human placenta on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human placenta identification?

Verified Customer

Verified customer

Asked: 2020-04-22

Answer

It shows on the product datasheet, M00199-1 anti-Wilms Tumor Protein Rabbit Monoclonal antibody has been validated for IF, IHC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human placenta in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-04-22

Question

I have a question about product M00199-1, anti-Wilms Tumor Protein Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-12-11

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00199-1 anti-Wilms Tumor Protein Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-12-11

Question

Will anti-Wilms Tumor Protein Rabbit Monoclonal antibody M00199-1 work on feline IHC with placenta?

Verified Customer

Verified customer

Asked: 2019-10-16

Answer

Our lab technicians have not validated anti-Wilms Tumor Protein Rabbit Monoclonal antibody M00199-1 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Wilms Tumor Protein Rabbit Monoclonal antibody M00199-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline placenta in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-10-16

Question

I see that the anti-Wilms Tumor Protein Rabbit Monoclonal antibody M00199-1 works with IF, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-06-26

Answer

You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-06-26

Question

I have attached the WB image, lot number and protocol we used for placenta using anti-Wilms Tumor Protein Rabbit Monoclonal antibody M00199-1. Please let me know if you require anything else.

N. Singh

Verified customer

Asked: 2019-05-20

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-05-20

Question

Is a blocking peptide available for product anti-Wilms Tumor Protein Rabbit Monoclonal antibody (M00199-1)?

Verified Customer

Verified customer

Asked: 2018-06-13

Answer

We do provide the blocking peptide for product anti-Wilms Tumor Protein Rabbit Monoclonal antibody (M00199-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-06-13

Question

We are currently using anti-Wilms Tumor Protein Rabbit Monoclonal antibody M00199-1 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2018-04-19

Answer

The anti-Wilms Tumor Protein Rabbit Monoclonal antibody (M00199-1) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-04-19

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
M00199-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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