Click image to see more details
Product Info Summary
| SKU: | A00199 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Wilms tumor protein WT1 Antibody
SKU/Catalog Number
A00199
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Wilms tumor protein WT1 Antibody catalog # A00199. Tested in WB,IF,IHC,ELISA applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Wilms tumor protein WT1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00199)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
KLH conjugated synthetic peptide derived from human WT1: 301-400/449
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00199 is reactive to WT1 in Human, Mouse, Rat
Calculated molecular weight
49.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00199 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2000
IHC: 1:100-1:500
IF: 1:100-1:500
ELISA: 1:5000-1:10000
Validation Images & Assay Conditions
Click image to see more details
Sample: TM4 Cell (Mouse) Lysate at 40 ug Primary: Anti-WT1 at 1/300 dilution
Click image to see more details
Sample: Testis (Mouse) Lysate at 40 ug Primary: Anti-WT1 at 1/300 dilution
Click image to see more details
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-WT1 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C
Click image to see more details
Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (WT1) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C.
Specific Publications For Anti-Wilms tumor protein WT1 Antibody (A00199)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Wilms tumor protein WT1 Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Wilms tumor protein WT1 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
