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- Table of Contents
WT1 antibody (A00199) performs well in ELISA, IF, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
Filter:
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-WT1 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C
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Anti-Wilms tumor protein WT1 Antibody
WT1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IF, IHC, WB |
Recommend Dilution
Application
Dilution
Species
WB: 1:500-1:2000
IHC: 1:100-1:500
IF: 1:100-1:500
ELISA: 1:5000-1:10000
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Wilms tumor protein WT1 Antibody
View all WT1 Antibodies
View all WT1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
KLH conjugated synthetic peptide derived from human WT1: 301-400/449
Cross-Reactivity
No cross reactivity with other proteins.
Calculated Molecular Weight
49.2 kDa
Cite This Product
Anti-Wilms tumor protein WT1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00199)
Publications
Application:
Species:
Dusabimana T,Park EJ,Je J,Jeong K,Yun SP,Kim HJ,Kim H,Park SW.Geniposide Improves Diabetic Nephropathy by Enhancing ULK1-Mediated Autophagy and Reducing Oxidative Stress through AMPK Activation.Int J Mol Sci.2021 Feb 6;22(4):1651.doi:10.3390/ijms22041651.
Species: Mouse
PMID: 33562139
Sun D, Bu L, Liu C, Yin Z, Zhou X, Li X, Xiao A. Plos One. 2013 May 28;8(5):E65042. Doi: 10.1371/Journal.Pone.0065042. Print 2013. Therapeutic Effects Of Human Amniotic Fluid-Derived Stem Cells On Renal Interstitial Fibrosis In A Murine Model Of U...
PMID: 23724119
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Product Promise
We promise Anti-Wilms tumor protein WT1 Antibody performs excellently in relevant applications for ELISA, IF, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A00199
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$405.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 13, 2026