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Product Info Summary
| SKU: | PB9063 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Wnt5a Antibody Picoband®
SKU/Catalog Number
PB9063
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Wnt5a Antibody Picoband® catalog # PB9063. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Wnt5a Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9063)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Wnt5a recombinant protein (Position: D184-D303). Human Wnt5a shares 100% amino acid (aa) sequence identity with both mouse and rat Wnt5a.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9063 is reactive to WNT5A in Human, Mouse, Rat
Observed Molecular Weight
45 kDa
Calculated molecular weight
42.3 kDa
Background of WNT5A
Protein Wnt-5a is a protein that in humans is encoded by the WNT5A gene. This gene is a member of the WNT gene family. The WNTs comprise a large class of secreted proteins that control essential developmental processes such as embryonic patterning, cell growth, migration, and differentiation. It is mapped to 3p14.3. WNT5A is highly expressed in the dermal papilla of depilated skin. This protein plays an essential role in regulating developmental pathways during embryogenesis. And this protein may also play a role in oncogenesis. WNT5A and DVL can promote axon differentiation mediated by the PAR3-PAR6-aPKC complex.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9063 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: human Hela whole cell, human PC-3 whole cell
IHC: human placenta tissue, human lung cancer tissue
Validation Images & Assay Conditions
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IHC analysis of Wnt5a using anti-Wnt5a antibody (PB9063).
Wnt5a was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Wnt5a Antibody (PB9063) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Western blot analysis of Wnt5a using anti-Wnt5a antibody (PB9063).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysate,
Lane 2: human PC-3 whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Wnt5a antigen affinity purified polyclonal antibody (Catalog # PB9063) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Wnt5a at approximately 45KD. The expected band size for Wnt5a is at 42KD.
Click image to see more details
IHC analysis of Wnt5a using anti-Wnt5a antibody (PB9063).
Wnt5a was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Wnt5a Antibody (PB9063) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Wnt5a Antibody Picoband® (PB9063)
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Customer Q&As
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5 Customer Q&As for Anti-Wnt5a Antibody Picoband®
Question
We bought anti-Wnt5a antibody for WB on placenta in the past. I am using rat, and We want to use the antibody for IHC next. I am interested in examining placenta as well as endometrium in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2019-12-17
Answer
I viewed the website and datasheets of our anti-Wnt5a antibody and I see that PB9063 has been validated on rat in both WB and IHC. Thus PB9063 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-12-17
Question
My question regards using your anti-Wnt5a antibody for negative regulation of canonical wnt signaling pathway studies. Has this antibody been tested with western blotting on human placenta tissue? We would like to see some validation images before ordering.
J. Evans
Verified customer
Asked: 2018-11-30
Answer
I appreciate your inquiry. This PB9063 anti-Wnt5a antibody is tested on human placenta tissue, hela whole cell lysate. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-11-30
Question
We are currently using anti-Wnt5a antibody PB9063 for rat tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2018-04-03
Answer
The anti-Wnt5a antibody (PB9063) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-04-03
Question
We have been able to see staining in rat placenta. Are there any suggestions? Is anti-Wnt5a antibody supposed to stain placenta positively?
Verified Customer
Verified customer
Asked: 2017-06-12
Answer
From what I have seen in literature placenta does express WNT5A. From what I have seen in Uniprot.org, WNT5A is expressed in endometrium, mesangial cell tongue, placenta, among other tissues. Regarding which tissues have WNT5A expression, here are a few articles citing expression in various tissues:
Mesangial cell, and Tongue, Pubmed ID: 14702039
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-06-12
Question
We were satisfied with the WB result of your anti-Wnt5a antibody. However we have seen positive staining in placenta secreted using this antibody. Is that expected? Could you tell me where is WNT5A supposed to be expressed?
M. Miller
Verified customer
Asked: 2016-03-24
Answer
From what I have seen in literature, placenta does express WNT5A. Generally WNT5A expresses in secreted, extracellular space, extracellular. Regarding which tissues have WNT5A expression, here are a few articles citing expression in various tissues:
Mesangial cell, and Tongue, Pubmed ID: 14702039
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2016-03-24
