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Product Info Summary
| SKU: | PA1218 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-XIAP-associated factor 1 XAF1 Antibody
SKU/Catalog Number
PA1218
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-XIAP-associated factor 1 XAF1 Antibody catalog # PA1218. Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-XIAP-associated factor 1 XAF1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1218)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human XAF1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1218 is reactive to XAF1 in Human
Applications
PA1218 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
34 kDa
Calculated molecular weight
34.626kDa
Background of XAF1
XIAP associated factor-1, also known as XAF1, is a human gene. X-linked inhibitor of apoptosis (XIAP; MIM 300079) is a potent member of the IAP family. All members of this family possess baculoviral IAP (BIR) repeats, cysteine-rich domains of approximately 80 amino acids that bind and inhibit caspases. XAF1 antagonizes the anticaspase activity of XIAP and may be important in mediating apoptosis resistance in cancer cells. And alteration in XAF1 and XIAP RNA expression levels may lead to increased apoptotic resistance and proliferation due to unregulated XIAP function in cancer cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
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Anti-XAF1 antibody, PA1218, IHC(P)
IHC(P): Human Endometrial Carcinoma Tissue
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Anti-XAF1 antibody, PA1218, Western blotting
All lanes: Anti XAF1 (PA1218) at 0.5ug/ml
Lane 1: HEPG2 Whole Cell Lysate at 40ug
Lane 2: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 34KD
Observed bind size: 34KD
Protein Target Info & Infographic
Gene/Protein Information For XAF1 (Source: Uniprot.org, NCBI)
Gene Name
XAF1
Full Name
XIAP-associated factor 1
Weight
34.626kDa
Alternative Names
BIRC4-binding protein; BIRC4BPBIRC4 binding protein; HSXIAPAF1; XIAP associated factor 1; XIAPAF1XIAP-associated factor 1 XAF1 BIRC4BP, HSXIAPAF1, XIAPAF1 XIAP associated factor 1 XIAP-associated factor 1|BIRC4-binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on XAF1, check out the XAF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for XAF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-XIAP-associated factor 1 XAF1 Antibody (PA1218)
Hello CJ!
No publications found for PA1218
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-XIAP-associated factor 1 XAF1 Antibody
Question
you antibody to test anti-XAF1 antibody PA1218 on human thymus for research purposes, then I may be interested in using anti-XAF1 antibody PA1218 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
The products we sell, including anti-XAF1 antibody PA1218, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-05-01
Question
I was wanting to use your anti-XAF1 antibody for IHC for human thymus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human thymus identification?
Verified Customer
Verified customer
Asked: 2020-01-16
Answer
It shows on the product datasheet, PA1218 anti-XAF1 antibody has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human thymus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-16
Question
We are currently using anti-XAF1 antibody PA1218 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-15
Answer
The anti-XAF1 antibody (PA1218) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-15
Question
Can you help my question with product PA1218, anti-XAF1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
E. Wu
Verified customer
Asked: 2014-10-29
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1218 anti-XAF1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-10-29
Question
I have attached the WB image, lot number and protocol we used for thymus using anti-XAF1 antibody PA1218. Please let me know if you require anything else.
L. Gonzalez
Verified customer
Asked: 2013-02-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-02-13
