Anti-Xanthine Oxidase/XDH Antibody Picoband®

Xanthine Oxidase antibody

Boster Bio Anti-Xanthine Oxidase/XDH Antibody Picoband® catalog # A01884. Tested in Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Cited in 1 publication(s).

Product Info Summary

SKU: A01884
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IHC, IHC-F, ICC, WB

Product Name

Anti-Xanthine Oxidase/XDH Antibody Picoband®

View all Xanthine Oxidase Antibodies

SKU/Catalog Number

A01884

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Xanthine Oxidase/XDH Antibody Picoband® catalog # A01884. Tested in Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Xanthine Oxidase/XDH Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01884)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2mg Na2HPO4, 0.05 mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human Xanthine Oxidase recombinant protein (Position: T2-K343). Human Xanthine Oxidase shares 86.8% and 89.2% amino acid (aa) sequence identity with mouse and rat Xanthine Oxidase, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01884 is reactive to XDH in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

146 kDa

Calculated molecular weight

146424 MW

Background of Xanthine Oxidase

Xanthine dehydrogenase, also known as XDH, is a protein that, in humans, is encoded by the XDH gene. Xanthine dehydrogenase belongs to the group of molybdenum-containing hydroxylases involved in the oxidative metabolism of purines. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Xanthine dehydrogenase can be converted to xanthine oxidase by reversible sulfhydryl oxidation or by irreversible proteolytic modification. Defects in xanthine dehydrogenase cause xanthinuria, may contribute to adult respiratory stress syndrome, and may potentiate influenza infection through an oxygen metabolite-dependent mechanism.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A01884 is guaranteed for Flow Cytometry, IHC, IHC-F, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, Human, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human
Immunocytochemistry, 0.5-1μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human

Positive Control

WB: rat liver tissue, mouse liver tissue
IHC: mouse intestine tissue, rat intestine tissue
FCM: A431 cell

Validation Images & Assay Conditions

Gene/Protein Information For XDH (Source: Uniprot.org, NCBI)

Gene Name

XDH

Full Name

Xanthine dehydrogenase/oxidase

Weight

146424 MW

Superfamily

xanthine dehydrogenase family

Alternative Names

Xanthine dehydrogenase/oxidase;Xanthine dehydrogenase;XD;1.17.1.4 ;Xanthine oxidase;XO;1.17.3.2 ;Xanthine oxidoreductase;XOR;XDH;XDHA; XDH XAN1, XO, XOR xanthine dehydrogenase xanthine dehydrogenase/oxidase|xanthine oxidoreductase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on XDH, check out the XDH Infographic

XDH infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for XDH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A01884 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Effect of Blueberry Anthocyanins Malvidin and Glycosides on the Antioxidant Properties in Endothelial Cells

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7 Customer Q&As for Anti-Xanthine Oxidase/XDH Antibody Picoband®

Question

Is a blocking peptide available for product anti-Xanthine Oxidase/XDH antibody (A01884)?

Verified Customer

Verified customer

Asked: 2020-04-16

Answer

We do provide the blocking peptide for product anti-Xanthine Oxidase/XDH antibody (A01884). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-04-16

Question

Can you help my question with product A01884, anti-Xanthine Oxidase/XDH antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-04-25

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01884 anti-Xanthine Oxidase/XDH antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-04-25

Question

My question regards to test anti-Xanthine Oxidase/XDH antibody A01884 on mouse milk for research purposes, then I may be interested in using anti-Xanthine Oxidase/XDH antibody A01884 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

W. Carter

Verified customer

Asked: 2018-07-13

Answer

The products we sell, including anti-Xanthine Oxidase/XDH antibody A01884, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-07-13

Question

I see that the anti-Xanthine Oxidase/XDH antibody A01884 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-05-02

Answer

You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-05-02

Question

Do you have a BSA free version of anti-Xanthine Oxidase/XDH antibody A01884 available?

Verified Customer

Verified customer

Asked: 2018-03-28

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Xanthine Oxidase/XDH antibody A01884 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-03-28

Question

We are currently using anti-Xanthine Oxidase/XDH antibody A01884 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?

N. Parker

Verified customer

Asked: 2015-09-29

Answer

The anti-Xanthine Oxidase/XDH antibody (A01884) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2015-09-29

Question

I was wanting to use your anti-Xanthine Oxidase/XDH antibody for Flow Cytometry for mouse milk on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse milk identification?

J. Roberts

Verified customer

Asked: 2014-12-26

Answer

As indicated on the product datasheet, A01884 anti-Xanthine Oxidase/XDH antibody has been tested for Flow Cytometry, IHC-P, IHC-F, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse milk in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2014-12-26

Order DetailsPrice
A01884

100μg

$370
A01884-10ug

10μg sample (liquid)

$99
A01884-Biotin

100 μg Biotin conjugated

$570
A01884-Cy3

100 μg Cy3 conjugated

$570
A01884-Dylight488

100 μg Dylight488 conjugated

$570
A01884-Dylight550

100 μg Dylight550 conjugated

$570
A01884-Dylight594

100 μg Dylight594 conjugated

$570
A01884-FITC

100 μg FITC conjugated

$570
A01884-HRP

100 μg HRP conjugated

$570
A01884-APC

100 μg APC conjugated

$670
A01884-PE

100 μg PE conjugated

$670
A01884-iFluor647

100 μg iFluor647 conjugated

$670
A01884-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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A01884
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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