Click image to see more details
Product Info Summary
| SKU: | M00234-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | ELISA, IF, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-XBP-1 Monoclonal Antibody [3H1G4]
SKU/Catalog Number
M00234-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-XBP-1 Monoclonal Antibody [3H1G4] (Catalog # M00234-1). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
XBP-1 monoclonal antibody can be stored at -20°C, stable for one year.
Cite This Product
Anti-XBP-1 Monoclonal Antibody [3H1G4] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00234-1)
Host
Mouse
Contents
XBP-1 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
Clone: 3H1G4
Isotype
IgG1
Immunogen
Mouse monoclonal XBP-1 antibody was raised against recombinant protein corresponding to amino acids 2 to 160 of human XBP-1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Biotin-GAPDH antibody is human, mouse, rat, and rabbit reactive.
Reactive Species
M00234-1 is reactive to XBP1 in Human, Mouse, Rat
Applications
M00234-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
28.695kDa
Background of XBP1
X box binding protein 1 (XBP-1) is a key protein in the mammalian unfolded protein response (UPR) that protects the cell against the stress of malfolded proteins in the endoplasmic reticulum (ER). Upon sensing unfolded proteins, an ER transmembrane endonuclease and kinase termed IRE1p is activated and excises an intron from XBP-1 mRNA. The spliced XBP-1 mRNA results in a 371 amino acid protein (XBP-1s) which is then translocated to the nucleus where it binds to the regulatory elements of downstream genes. Together with other UPR transcription factors such as ATF6, XBP-1 stimulates the production of ER stress proteins including the ER resident protein chaperones glucose regulated protein (GRP) 78 and GRP94.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
XBP-1 antibody can be used for the detection of XBP-1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 4 μg/mL.
Antibody validated: Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of 100 ng of XBP-1 recombinant protein with XBP-1 antibody at 1 μg/mL.
Click image to see more details
Immunocytochemistry of XBP-1 in HepG2 cells with XBP-1 antibody at 2 μg/mL.
Click image to see more details
Immunofluorescence of XBP1 in HepG2 cells with XBP1 antibody at 4 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For XBP1 (Source: Uniprot.org, NCBI)
Gene Name
XBP1
Full Name
X-box-binding protein 1
Weight
28.695kDa
Superfamily
bZIP family
Alternative Names
HTF; TREB5; X-box binding protein 1; X-box-binding protein 1; XBP1; XBP-1; XBP2; XBP2Tax-responsive element-binding protein 5 XBP1 TREB-5, TREB5, XBP-1, XBP2 X-box binding protein 1 X-box-binding protein 1|tax-responsive element-binding protein 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on XBP1, check out the XBP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for XBP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-XBP-1 Monoclonal Antibody [3H1G4] (M00234-1)
Hello CJ!
No publications found for M00234-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-XBP-1 Monoclonal Antibody [3H1G4]?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-XBP-1 Monoclonal Antibody [3H1G4]
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
