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Product Info Summary
| SKU: | M00234 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-XBP1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00234
BM4993 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-XBP1 Rabbit Monoclonal Antibody catalog # M00234. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-XBP1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00234)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AAAF-24
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human XBP1
Reactive Species
M00234 is reactive to XBP1 in Human
Observed Molecular Weight
35 kDa
Calculated molecular weight
28.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00234 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:500
ICC/IF 1:50-1:200
FC 1:50
Validation Images & Assay Conditions
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Western blot analysis of XBP1 expression in Jurkat cell lysate.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Specific Publications For Anti-XBP1 Rabbit Monoclonal Antibody (M00234)
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5 Customer Q&As for Anti-XBP1 Rabbit Monoclonal Antibody
Question
We are currently using anti-XBP1 Rabbit Monoclonal antibody M00234 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
The anti-XBP1 Rabbit Monoclonal antibody (M00234) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-29
Question
I am interested in using your anti-XBP1 Rabbit Monoclonal antibody for ubiquitin-dependent protein catabolic process studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
Thank you for your inquiry. This M00234 anti-XBP1 Rabbit Monoclonal antibody is tested on jurkat cell lysate. It is guaranteed to work for IF, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-11-15
Question
We ordered your anti-XBP1 Rabbit Monoclonal antibody for WB on ovary placenta in a previous experiment. I am using human, and We are going to use the antibody for IF next. you antibody examining ovary placenta as well as b-cell in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
I have checked the website and datasheets of our anti-XBP1 Rabbit Monoclonal antibody and I see that M00234 has been validated on human in both WB and IF. Thus M00234 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-07-04
Question
We have seen staining in human ovary placenta. Do you have any suggestions? Is anti-XBP1 Rabbit Monoclonal antibody supposed to stain ovary placenta positively?
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
Based on literature ovary placenta does express XBP1. Based on Uniprot.org, XBP1 is expressed in body of pancreas, b-cell, ovary placenta, erythroleukemia, among other tissues. Regarding which tissues have XBP1 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 2321018
Erythroleukemia, Pubmed ID: 23186163
Ovary, and Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-05-24
Question
My team were happy with the WB result of your anti-XBP1 Rabbit Monoclonal antibody. However we have observed positive staining in erythroleukemia x-box-binding protein 1 using this antibody. Is that expected? Could you tell me where is XBP1 supposed to be expressed?
M. Li
Verified customer
Asked: 2014-11-28
Answer
Based on literature, erythroleukemia does express XBP1. Generally XBP1 expresses in endoplasmic reticulum, isoform 1: nucleus, isoform 2: nucleus, x-box-binding protein 1, cytoplasmic form:. Regarding which tissues have XBP1 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 2321018
Erythroleukemia, Pubmed ID: 23186163
Ovary, and Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2014-11-28
